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Marcello Massimini

30 papers in the library · 7,468 citations · publishing 2005-2025

Papers

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Thermodynamics of consciousness: A non-invasive perturbational framework

bioRxiv Preprint Server December 9, 2025 Tomas Berjaga-Buisan, Juan Manuel Monti, Martina Cortada et al. preprint

The quest for reliable and objective measures of consciousness is critical in basic and clinical neuroscience. Across species, the Perturbational Complexity Index (PCI) has emerged as a robust empirical marker by directly perturbing the brain, yet its underlying principles of physics are not fully understood. Here, we bridge this gap by introducing a non-invasive framework based on generative...

Multiscale dynamical characterization of cortical brain states: from synchrony to asynchrony

arXiv Preprint Archive October 7, 2025 Maria V. Sanchez-Vives, Arnau Manasanch, Andrea Pigorini et al.

The cerebral cortex spontaneously displays different patterns of activity that evolve over time according to the brain state. Sleep, wakefulness, resting states, and attention are examples of a wide spectrum of physiological states that can be sustained by the same structural network. Furthermore, additional states are generated by drugs (e.g., different levels of anesthesia) or by pathological...

Hemispherotomy leads to persistent sleep-like slow waves in the isolated cortex of awake humans.

PLoS Biology October 1, 2025 Michele Angelo Colombo, Jacopo Favaro, Ezequiel Mikulan et al. 7 citations

Hemispherotomy is a neurosurgical procedure for treating refractory epilepsy, which entails disconnecting a significant portion of the cortex, potentially encompassing an entire hemisphere, from its cortical and subcortical connections. While this intervention prevents the spread of seizures, it raises important questions. Given the complete isolation from sensory-motor pathways, it remains...

Conscious tactile perception entails distinct neural dynamics within somatosensory areas.

Current biology : CB June 9, 2025 Davide Albertini, Maria Del Vecchio, Ivana Sartori et al. 9 citations

Distilling the neural correlates of consciousness (NCCs) in humans is challenging due to limitations in the spatiotemporal resolution of recording techniques and confounds related to pre- and post-perceptual processes. In this study, we leveraged the detailed insights provided by human intracortical recordings to elucidate how somatosensory responses to simple tactile stimuli vary across...

An integrative, multiscale view on neural theories of consciousness.

Neuron May 15, 2024 Johan F. Storm, P Christiaan Klink, Jaan Aru et al. 108 citations

How is conscious experience related to material brain processes? A variety of theories aiming to answer this age-old question have emerged from the recent surge in consciousness research, and some are now hotly debated. Although most researchers have so far focused on the development and validation of their preferred theory in relative isolation, this article, written by a group of scientists...

Tests for consciousness in humans and beyond.

Trends in Cognitive Sciences March 1, 2024 Tim Bayne, Anil K. Seth, Marcello Massimini et al.

Which systems/organisms are conscious? New tests for consciousness ('C-tests') are urgently needed. There is persisting uncertainty about when consciousness arises in human development, when it is lost due to neurological disorders and brain injury, and how it is distributed in nonhuman species. This need is amplified by recent and rapid developments in artificial intelligence (AI), neural...

Spatiotemporal brain complexity quantifies consciousness outside of perturbation paradigms

bioRxiv Preprint Server April 18, 2023 Martin Breyton, Jan Fousek, Giovanni Rabuffo et al. preprint

Signatures of consciousness are found in spectral and temporal properties of neuronal activity. Among these, spatiotemporal complexity after a perturbation has recently emerged as a robust metric to infer levels of consciousness. Perturbation paradigms remain, however, difficult to perform routinely. To discover alternative paradigms and metrics, we systematically explore brain stimulation and...

Quantifying arousal and awareness in altered states of consciousness using interpretable deep learning

Nature Communications February 25, 2022 Minji Lee, Leandro R. D. Sanz, Alice Barra et al. 120 citations

Consciousness can be defined by two components: arousal (wakefulness) and awareness (subjective experience). However, neurophysiological consciousness metrics able to disentangle between these components have not been reported. Here, we propose an explainable consciousness indicator (ECI) using deep learning to disentangle the components of consciousness. We employ electroencephalographic (EEG)...

Consciousness and complexity: a consilience of evidence

Neuroscience of Consciousness 2021 Simone Sarasso, Adenauer G. Casali, Silvia Casarotto et al. 183 citations

Abstract Over the last years, a surge of empirical studies converged on complexity-related measures as reliable markers of consciousness across many different conditions, such as sleep, anesthesia, hallucinatory states, coma, and related disorders. Most of these measures were independently proposed by researchers endorsing disparate frameworks and employing different methods and techniques....

Are There Islands of Awareness?

Trends in Neurosciences December 10, 2019 Tim Bayne, Anil K. Seth, Marcello Massimini 146 citations

Awareness may persist in fully disconnected cortical islands.We identify both natural and artificial examples of potential islands of awareness.Detecting islands of awareness poses difficult but often addressable challenges.The possibility of islands of awareness raises important ethical and legal issues.The discovery of islands of awareness would have important implications for debates about...

Fractal dimension analysis of states of consciousness and unconsciousness using transcranial magnetic stimulation

Comput. Methods Programs Biomed. July 1, 2019 J. R. D. Miras, Fernando Soler-Toscano, Sergio Iglesias-Parro et al.

BACKGROUND AND OBJECTIVE Knowing whether a subject is conscious or not is a current challenge with a deep potential clinical impact. Recent theoretical considerations suggest that consciousness is linked to the complexity of distributed interactions within the corticothalamic system. The fractal dimension (FD) is a quantitative parameter that has been extensively used to analyse the complexity...

The spectral exponent of the resting EEG indexes the presence of consciousness during unresponsiveness induced by propofol, xenon, and ketamine

Neuroimage January 11, 2019 Michele A. Colombo, Martino Napolitani, Melanie Boly et al. 359 citations

Despite the absence of responsiveness during anesthesia, conscious experience may persist. However, reliable, easily acquirable and interpretable neurophysiological markers of the presence of consciousness in unresponsive states are still missing. A promising marker is based on the decay-rate of the power spectral density (PSD) of the resting EEG. We acquired resting electroencephalogram (EEG)...

Meditation-induced modulation of brain response to transcranial magnetic stimulation.

Brain Stimulation November 1, 2018 Olivier Bodart, Matteo Fecchio, Marcello Massimini et al.

Meditation can be conceptualized as a family of complex emotional and attentional regulatory training regimes developed for various ends, including the cultivation of well-being and emotional balance. Previous studies have demonstrated that meditation may have positive effects on self-awareness, emotion regulation, attention processes, and also structural neuroplasticity [1]. However, the...

Cerebral organoids: ethical issues and consciousness assessment.

Journal of Medical Ethics September 1, 2018 Andrea Lavazza, Marcello Massimini

Organoids are three-dimensional biological structures grown in vitro from different kinds of stem cells that self-organise mimicking real organs with organ-specific cell types. Recently, researchers have managed to produce human organoids which have structural and functional properties very similar to those of different organs, such as the retina, the intestines, the kidneys, the pancreas, the...

Sizing up Consciousness

Oxford Scholarship Online May 30, 2018 Marcello Massimini, Giulio Tononi

Sizing up Consciousness explores, at an introductory level, the potential practical, clinical, and ethical implications of a general principle about the nature of consciousness. Using information integration theory (IIT) as a guiding principle, the book takes the reader along a scientific trajectory to face fundamental questions about the relationships between matter and experience. What is so...

Consciousness Regained: Disentangling Mechanisms, Brain Systems, and Behavioral Responses.

The Journal of neuroscience : the official journal of the Society for Neuroscience November 8, 2017 Johan F. Storm, Melanie Boly, Adenauer G. Casali et al.

How consciousness (experience) arises from and relates to material brain processes (the "mind-body problem") has been pondered by thinkers for centuries, and is regarded as among the deepest unsolved problems in science, with wide-ranging theoretical, clinical, and ethical implications. Until the last few decades, this was largely seen as a philosophical topic, but not widely accepted in...

Are the Neural Correlates of Consciousness in the Front or in the Back of the Cerebral Cortex? Clinical and Neuroimaging Evidence

Journal of Neuroscience October 4, 2017 Melanie Boly, Marcello Massimini, Naotsugu Tsuchiya et al. 591 citations

The role of the frontal cortex in consciousness remains a matter of debate. In this Perspective, we will critically review the clinical and neuroimaging evidence for the involvement of the front versus the back of the cortex in specifying conscious contents and discuss promising research avenues. Dual Perspectives Companion Paper: Should a Few Null Findings Falsify Prefrontal Theories of...

Global and local complexity of intracranial EEG decreases during NREM sleep

Neuroscience of Consciousness January 27, 2017 Michael Schartner, Andrea Pigorini, Steve A. Gibbs et al. 158 citations

Key to understanding the neuronal basis of consciousness is the characterization of the neural signatures of changes in level of consciousness during sleep. Here we analysed three measures of dynamical complexity on spontaneous depth electrode recordings from 10 epilepsy patients during wakeful rest (WR) and different stages of sleep: (i) Lempel-Ziv complexity, which is derived from how...

Stratification of unresponsive patients by an independently validated index of brain complexity

Annals of Neurology September 22, 2016 Silvia Casarotto, Angela Comanducci, Mario Rosanova et al. 504 citations

OBJECTIVE: Validating objective, brain-based indices of consciousness in behaviorally unresponsive patients represents a challenge due to the impossibility of obtaining independent evidence through subjective reports. Here we address this problem by first validating a promising metric of consciousness-the Perturbational Complexity Index (PCI)-in a benchmark population who could confirm the...

Consciousness and cortical responsiveness: a within-state study during non-rapid eye movement sleep

Scientific Reports August 5, 2016 Jaakko O. Nieminen, Olivia Gosseries, Marcello Massimini et al. 60 citations

When subjects become unconscious, there is a characteristic change in the way the cerebral cortex responds to perturbations, as can be assessed using transcranial magnetic stimulation and electroencephalography (TMS-EEG). For instance, compared to wakefulness, during non-rapid eye movement (NREM) sleep TMS elicits a larger positive-negative wave, fewer phase-locked oscillations, and an overall...

Neural correlates of consciousness: progress and problems

Nature reviews. Neuroscience April 20, 2016 Christof Koch, Marcello Massimini, Melanie Boly et al. 1,654 citations

There have been a number of advances in the search for the neural correlates of consciousness--the minimum neural mechanisms sufficient for any one specific conscious percept. In this Review, we describe recent findings showing that the anatomical neural correlates of consciousness are primarily localized to a posterior cortical hot zone that includes sensory areas, rather than to a...

Stimulus Set Meaningfulness and Neurophysiological Differentiation: A Functional Magnetic Resonance Imaging Study

PLoS One May 13, 2015 Melanie Boly, Shuntaro Sasai, Olivia Gosseries et al. 69 citations

A meaningful set of stimuli, such as a sequence of frames from a movie, triggers a set of different experiences. By contrast, a meaningless set of stimuli, such as a sequence of 'TV noise' frames, triggers always the same experience--of seeing 'TV noise'--even though the stimuli themselves are as different from each other as the movie frames. We reasoned that the differentiation of cortical...

Transcranial magnetic stimulation combined with high-density EEG in altered states of consciousness

Brain Injury August 1, 2014 Martino Napolitani, Olivier Bodart, Paola Canali et al. 44 citations

BACKGROUND: This review discusses the advantages of transcranial magnetic stimulation combined with high-density electroencephalography (TMS-hdEEG) over other current techniques of brain imaging. METHODS AND RESULTS: Its application was reviewed, focusing particularly on disorders of consciousness, in the perspective of recent theories of consciousness. Assessment of non-communicative patients...

Quantifying Cortical EEG Responses to TMS in (Un)consciousness

Clinical EEG and Neuroscience 2014 Simone Sarasso, Mario Rosanova, Adenauer G. Casali et al. 164 citations

We normally assess another individual's level of consciousness based on her or his ability to interact with the surrounding environment and communicate. Usually, if we observe purposeful behavior, appropriate responses to sensory inputs, and, above all, appropriate answers to questions, we can be reasonably sure that the person is conscious. However, we know that consciousness can be entirely...

A Theoretically Based Index of Consciousness Independent of Sensory Processing and Behavior

Science Translational Medicine August 14, 2013 Adenauer G. Casali, Olivia Gosseries, Mario Rosanova et al. 1,299 citations

One challenging aspect of the clinical assessment of brain-injured, unresponsive patients is the lack of an objective measure of consciousness that is independent of the subject's ability to interact with the external environment. Theoretical considerations suggest that consciousness depends on the brain's ability to support complex activity patterns that are, at once, distributed among...