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bioRxiv (Cold Spring Harbor Laboratory)

205 papers in the library · 584 citations · publishing 2015-2026

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Long-timescale dynamics shape visual conscious awareness

bioRxiv (Cold Spring Harbor Laboratory) September 8, 2026 Zefan Zheng, Darinka Trübutschek, Yongchun Cai et al. preprint

Abstract Consciousness is mostly studied as if it were composed of independent snapshots, aggregating reports over trials and experimental contexts, disregarding any temporal aspect. However, almost nothing is known about how conscious experience spontaneously fluctuates in time. To shed light on the temporal dynamics of conscious awareness, we evaluated the temporal autocorrelation of...

Dose-dependent effect of ketamine on functional connectivity in mice

bioRxiv (Cold Spring Harbor Laboratory) September 8, 2026 Tomokazu Tsurugizawa, Aya Takemura preprint

A single injection of a subanesthetic dose of ketamine, an NMDA receptor antagonist, induces schizophrenia-like behavior in mice. Previous studies investigated the effect of single injection of ketamine on behavior and c-Fos expression in mice dose-dependently. However, there has not been functional magnetic resonance imaging (fMRI) study to investigate the effect of ketamine in mice. Here, we...

Ketamine Modulates Endogenous Opioid Peptide Signaling and Reduces Heroin-Seeking in Male Long-Evans Rats

bioRxiv (Cold Spring Harbor Laboratory) September 6, 2026 Adam M Dawoud, Achla Gupta, Ivone Gomes et al. preprint

Opioid Use Disorder (OUD) continues to be a pressing public health crisis, marked by high relapse rates and limited treatment options. Ketamine, a non-competitive N-Methyl-D-Aspartate receptor (NMDAR) antagonist, has emerged as a promising therapeutic for numerous psychiatric illnesses due to its robust antidepressant properties and glutamate-mediated synaptic plasticity. However, ketamine's...

Preserved brain hierarchy supports residual vision without awareness after visual cortex damage

bioRxiv (Cold Spring Harbor Laboratory) September 1, 2026 Davide Orsenigo, Andrea I. Luppi, Matteo Diano et al. preprint

Damage to the primary visual cortex causes loss of conscious vision, yet some patients retain the ability to respond to stimuli despite reporting no visual experience. Why similar lesions produce such different behavioral phenotypes remains unclear. While research to date has focused primarily on spared pathways that bypass V1, here we asked whether these divergent outcomes are also linked to...

Multimodal Ganzfeld-induced visual experiences are associated with alongside mental experiences and distinct EEG microstate dynamics

bioRxiv (Cold Spring Harbor Laboratory) August 31, 2026 Xinlin Wang, Yannick Pomorin, Emma Peters et al. preprint

Abstract During wakefulness, we are used to perceive the environment through our senses, act on it and take these inputs to update our experiences and build the perceptions. When the inputs are not longer accurate or structured, people would sometimes have hallucinatory experiences. Whether such experiences are associated with distinct patterns of thought, and how they relate to large-scale...

A dominant frontoparietal beta oscillatory brain state in the days after psilocybin and 5-MeO-DMT

bioRxiv (Cold Spring Harbor Laboratory) August 27, 2026 Chloe L. West, Bradley Baker, Annabel Duran et al. preprint

Abstract Background Serotonergic psychedelics show promise for treating psychiatric disorders, with symptom improvements lasting for weeks after a single dose. Clarifying the neural basis of these effects would benefit from an identification of empirical biomarkers of such lasting shifts in brain function. Resting state EEG offers a rapid (<5 minute), low-cost window into functional brain...

Psilocybin collapses visual change detection and drives cortical dynamics toward a state of surprise

bioRxiv (Cold Spring Harbor Laboratory) August 25, 2026 Roberto de Filippo, Ryan Gillis, David G Wyrick et al. preprint

Abstract Psilocybin profoundly alters visual perception, yet the neuronal mechanisms underlying these effects remain unclear. Here we combined large-scale Neuropixels recordings with cell-type-specific optogenetics in head-fixed mice performing a visual change-detection task. Psilocybin severely impaired task performance without overt motor deficits. In cortex, the drug modestly suppressed...

Neural Signatures of Conscious Experience During Sleep: A Serial Awakening Study Using High-Density EEG

bioRxiv (Cold Spring Harbor Laboratory) August 20, 2026 Atakan Selte, Steven E. Haworth, Thomas J. Vanasse et al. preprint

Abstract Identifying neural signatures of consciousness remains a central challenge in neuroscience. Sleep offers a tractable model for comparing brain activity in the presence or absence of subjective experience while minimizing behavioral responsiveness confounds. Using overnight 256-electrode high-density EEG in 140 participants and a serial-awakening paradigm, we analyzed 699 non-rapid eye...

Persisting perceptual abnormalities in psychedelic users are associated with conditioned hallucinations and impaired sensory processing

bioRxiv (Cold Spring Harbor Laboratory) August 16, 2026 Maximillian Greenwald, Peter Thestrup Waade, Eren Kafadar et al. preprint

Abstract Serotonergic psychedelics (SP) are increasingly used in clinical research and naturalistic settings, but their psychotic-like side effects, including persisting perceptual abnormalities (PPAs), are poorly understood. Psychosis-associated hallucinations are associated with susceptibility to conditioned hallucinations and computationally-estimated overweighting of perceptual...

Preclinical Comparison of DMT and 5-MeO-DMT Reveals Behavioral Dissociation, Distinct TrkB Activation and Differential Plasticity Profiles

bioRxiv (Cold Spring Harbor Laboratory) August 12, 2026 Orr Shahar, Alexander Botvinnik, Masha Chaykin et al. preprint

Abstract N, N-dimethyltryptamine (DMT) and 5-methoxy-N, N-dimethyltryptamine (5-MeO-DMT) are structurally related tryptamine psychedelics with emerging therapeutic potential, yet their comparative acute pharmacology and longer-term neuroplastic effects remain incompletely defined. Here we show that DMT produces a bell-shaped dose–response curve in the mouse head-twitch response (HTR) assay,...

MDMA-Enhanced Exposure Therapy Reverses PTSD-Like Features In a Learned Helplessness Mouse Model

bioRxiv (Cold Spring Harbor Laboratory) August 12, 2026 Orr Shahar, Perettz Golding, Masha Chaykin et al. preprint

Abstract Post-traumatic stress disorder (PTSD) is a highly prevalent, debilitating psychiatric condition. Existing treatments are ineffective for many patients. 3,4- methylenedioxymethamphetamine (MDMA)-assisted psychotherapy has demonstrated substantial clinical efficacy but relies on prolonged, resource-intensive therapeutic protocols that limit scalability and accessibility. Here, we...

Direct anti-inflammatory actions of N,N -dimethyltryptamine on microglia are revealed by proteomic profiling and receptor pharmacology

bioRxiv (Cold Spring Harbor Laboratory) August 11, 2026 István Pesti, Ágnes Bessenyei, Rita Frank et al. preprint

ABSTRACT N,N -dimethyltryptamine (DMT) is an endogenous psychedelic tryptamine that has recently emerged as a promising therapeutic candidate for acute ischemic stroke. Although DMT consistently reduces infarct size, attenuates neuroinflammation, and improves functional outcome in experimental stroke, the cellular and receptor mechanisms underlying these effects remain poorly understood....

Circulating MicroRNAs and Their Associations with Neurotrophic, Inflammatory and Glutamate Markers in Healthy Volunteers

bioRxiv (Cold Spring Harbor Laboratory) August 10, 2026 Aedan O'Shea, Natasha L. Mason, Jacco Briede et al. preprint

Abstract Psilocybin acutely alters neurotrophic, neurochemical, and immune markers, but the relationships between these responses and circulating microRNAs (miRNAs), i.e. non-coding RNAs that regulate post-transcriptional gene expression, remain unclear. In a randomized, double-blind, placebo-controlled study of 62 healthy adults who received psilocybin (0.17 mg/kg) or placebo, we previously...

Flattened brain hierarchy and increased EEG complexity during unusual bodily experiences and out-of-body experiences

bioRxiv (Cold Spring Harbor Laboratory) August 4, 2026 Teresa Campillo-Ferrer, Tomas Berjaga-Buisan, Antonella Iadarola et al. preprint

Abstract Experiences in which the sense of the body becomes distorted offer a unique window into how the brain constructs the bodily self. Despite their clinical relevance in conditions such as depersonalization and body-image disorders, large-scale brain dynamics underlying unusual bodily experiences (UBEs) remain poorly understood, and no prior study has examined them systematically across...

Mind-manifesting? Examining naturalistic reports from psychedelics, cannabis, meditation, and dreams from the ALTERD mobile application

bioRxiv (Cold Spring Harbor Laboratory) August 4, 2026 Conor H. Murray, Michael Angyus, Sam Suchin et al. preprint

Abstract Altered states of consciousness (ASC) refer to states that differ markedly from normal waking consciousness. ASC may occur spontaneously, such as in dreaming, or through consciousness modifying techniques, from meditation, to cannabis and psychedelics. The etymology behind the term psychedelic, meaning mind-manifesting, is based on the hypothesis that certain substances, including...

The psychedelic DOI decreases the modularity of the cortical network by increasing cortico-cortical and thalamo-cortical functional influence

bioRxiv (Cold Spring Harbor Laboratory) July 24, 2026 Roman Doronin, Eduard Stroukov, Georg B. Keller preprint

SUMMARY Psychedelics are powerful modulators of conscious perception, hypothesized to exert their effects by reorganizing large-scale brain functional connectivity. The circuit mechanisms underlying this reorganization remain unknown. Here, using widefield calcium imaging in awake mice, we found that the psychedelic DOI, in a 5-HT2A-dependent manner, acutely decreased the modularity of the...

Psychedelic drug action at dendrites is gated by behavioral state and serotonin receptors

bioRxiv (Cold Spring Harbor Laboratory) July 20, 2026 Neil K. Savalia, Ling-Xiao Shao, Cory A. Knox et al.

SUMMARY How psychedelics act on cortical dendrites to produce long-lasting structural plasticity remains poorly understood. Here, we characterize the effects of psilocybin on dendritic calcium dynamics in pyramidal tract neurons of the mouse medial frontal cortex. Psilocybin transiently increases calcium event rates in apical dendritic tufts over a time course that parallels the drug’s...

Autonomic challenge uncovers hidden link between diastolic blood pressure and mental imagery vividness

bioRxiv (Cold Spring Harbor Laboratory) July 17, 2026 Yoko Nagai

Mental imagery is viewed as a fundamental component of human cognition, supporting memory, emotional processing, future simulation, and conscious experience. Although mental imagery vividness has traditionally been attributed to differences in sensory processing, emerging evidence suggests that internally generated images are also shaped by ongoing bodily and interoceptive signals. Here, we...

Neural correlates of the subjective experience of free-will during value-based risky decisions: a pilot study

bioRxiv (Cold Spring Harbor Laboratory) July 17, 2026 Priyamvada Modak, Joshua W. Brown

Underlying the very notion of choice is the fundamental idea of free-will, which is challenged by decision-neuroscience aiming to explain and predict choices using interactions of neurons. The question of whether any choice is truly a free-choice and born out of free-will has long been a subject of philosophical debate. In this work, we do not take a position on this debate, rather...

Uncovering the spatiotemporal structure of neural avalanches through optimal transport and dynamic time warping

bioRxiv (Cold Spring Harbor Laboratory) July 16, 2026 Filip Novický, Nikola Jajcay, Jaroslav Hlinka

Neural avalanches - or threshold-defined bursts of coordinated activity - are traditionally characterised by scale-free statistics. However the study of their detailed spatiotemporal structure and their recurrence within spontaneous activity has been hindered by the variability of avalanches in duration and spatial extent. To tackle this challenge, we propose the use of flexible alignment: we...

Sex Differences in Acute Responses to Psychedelics: Evidence for Greater Subjective Intensity and Impairment in Female Participants

bioRxiv (Cold Spring Harbor Laboratory) July 13, 2026 Natasha L. Mason, Eline Chm Haijen-Bongers, Kim P. C. Kuypers et al.

Serotonergic psychedelics are advancing as psychiatric treatments, yet acute responses vary between individuals and the contribution of sex, a fundamental biological variable, remains largely unexamined. We pooled two double-blind, placebo-controlled studies in healthy volunteers (N = 72; 31 male, 41 female) comparing psilocybin 15 mg, 2C-B 20 mg, and LSD 50 μg. Linear mixed models tested sex...

Dopamine Compensates for Amyloid-Induced Default Mode Network Dysfunction to Support Learning

bioRxiv (Cold Spring Harbor Laboratory) July 10, 2026 Joseph Giorgio, Thomas Morin, Hsiang-Yu Chen et al.

Throughout the preclinical phase of Alzheimer's disease (AD) β-amyloid (Aβ) accumulates preferentially within the default mode network (DMN), yet the functional and behavioural consequences of this pathological burden remain poorly understood. Using task-based fMRI combined with Aβ, tau, and dopamine PET in cognitively normal older adults, we show that Aβ burden impairs learning independent of...

Ligand-specific effects of 5-HT2A receptor antagonists on fear extinction in C57BL/6J mice: Comparative insights from MDL 11,939 and MDL 100,907

bioRxiv (Cold Spring Harbor Laboratory) July 3, 2026 Anastasia Tyulmenkova, Robert W. Stackman

Abstract Serotonin (5-HT) 2A receptors (5-HT 2 AR) modulate corticolimbic circuits regulating fear extinction. Although activation of these receptors has been shown to facilitate fear extinction, the behavioral consequences of 5-HT 2 AR antagonism during extinction is not well defined. Here, we examined the systemic effects of two 5-HT 2 A receptor antagonists, the mixed 5-HT 2 A/ 2 C...

Nondual mindfulness meditation alters self representation and brain connectome in expert meditators

bioRxiv (Cold Spring Harbor Laboratory) July 3, 2026 Sébastien Czajko, Jelle Zorn, Oussama Abdoun et al.

Nonduality is a foundational but conceptually elusive notion across several contemplative traditions. Nondual traditions challenge the assumption that a subject-object structure characteristic of ordinary experience is an intrinsic feature of conscious awareness. However, little remains known about the neurocognitive mechanisms associated with such experiential state. Here, we investigated how...

Electroencephalogram recordings in Jhana states: An open dataset

bioRxiv (Cold Spring Harbor Laboratory) July 2, 2026 Marco S. Fabus, Stephen Zerfas, Alex Gruver et al.

Abstract The use of meditation as a tool to improve human wellbeing is receiving considerable scientific interest. However, most existing research has focused on concentration-based practices. One powerful alternative is jhana meditation, which leads to states characterised by self-reinforcing bliss, potentially useful for a variety of clinical and scientific domains. However, our understanding...