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Eugenia Gallardo

19 papers in the library · 366 citations · publishing 2016-2025

Papers

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Analysis of drugs of abuse and contaminants in individuals under drug surveillance programs: A study in hair samples.

Forensic Science International June 1, 2025 Ana Y. Simão, P Y Tokuyama, G Zampieri et al. 1 citation

In this study, over 900 hair samples from individuals using drugs of abuse in São Paulo and Rio Grande do Sul, Brazil were analysed for classical drugs and new psychoactive substances (NPS) in hair samples. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods were developed for the detection of some drugs (25B-NBOMe, 25C-NBOMe, 25E-NBOH, 2C-C, 2C-I, 3,4-MDPHP, 3-MeO-PCP,...

Comparative study of sample preparation procedures to determine the main compounds in ayahuasca beverages by QuEChERS and high-performance liquid chromatography analysis.

Phytochemical analysis : PCA August 1, 2024 Joana Gonçalves, Tiago Rosado, Mário Barroso et al. 2 citations

Ayahuasca is a psychoactive drink originally consumed by indigenous people of the Amazon. The lack of regulation of this drink leads to uncontrolled consumption, and it is often consumed in religious contexts. The aim of this work is to compare three miniaturised extraction techniques for extracting the main ayahuasca compounds from beverages. Three sample pretreatment techniques were evaluated...

Determination of Arylcyclohexylamines in Biological Specimens: Sensors and Sample Pre-Treatment Approaches.

Micromachines July 30, 2024 Rodrigo Pelixo, Mário Barroso, Eugenia Gallardo et al. 1 citation

Arylcyclohexylamine (ACH) compounds represent a predominant faction within new psychoactive substances. Due to their powerful dissociative effects, they are used in recreational contexts but also in situations of drug-facilitated sexual assault, and therefore, they are a constant target of analysis by forensic experts. In recent years, their consumption has been notably high, especially the use...

The role of ayahuasca in cell viability and oxidative stress in gastric adenocarcinoma cell line.

Natural Product Research July 4, 2024 Joana Gonçalves, José Francisco Cascalheira, Patrícia Valentão et al.

Ayahuasca, a psychoactive beverage native to the Amazon, originally derived from Banisteriopsis caapi stem scrapings and Psychotria viridis leaves, exhibits hallucinogenic properties due to N,N-dimethyltryptamine. When combined with β-carbolines, it enters the bloodstream and central nervous system, inhibiting monoamine oxidase-A. Over time, therapeutic effects have been associated to ayahuasca...

The Role of Ayahuasca in Colorectal Adenocarcinoma Cell Survival, Proliferation and Oxidative Stress.

Pharmaceuticals (Basel, Switzerland) June 2, 2024 Joana Gonçalves, Mariana Feijó, Sílvia Socorro et al.

The psychedelic beverage ayahuasca is originally obtained by Banisteriopsis caapi (B. caapi) (BC) and Psychotria viridis (P. viridis) (PV). However, sometimes these plant species are replaced by others that mimic the original effects, such as Mimosa hostilis (M. hostilis) (MH) and Peganum harmala (P. harmala) (PH). Its worldwide consumption and the number of studies on its potential therapeutic...

The Therapeutic Potential of Amphetamine-like Psychostimulants

Life November 8, 2023 Bruno Pires, Luana M. Rosendo, Ana Teresa Brinca et al. 15 citations

This review delves into the therapeutic applications of amphetamine-type stimulants such as lisdexamphetamine dimesylate, mixed amphetamine salts, 3,4-methylenedioxymethamphetamine (MDMA), dextroamphetamine, and phentermine. These compounds have been investigated for their potential in treating a range of psychiatric disorders, including attention deficit hyperactivity disorder (ADHD), drug...

A Systematic Review on the Therapeutic Effects of Ayahuasca.

Plants (Basel, Switzerland) July 7, 2023 Joana Gonçalves, Ângelo Luís, Eugenia Gallardo et al. 16 citations

Traditional therapies, resorting to the use of plants, have acquired a great demand over the years, both for economic reasons and the preference for natural treatments. Some studies suggest that ayahuasca may have beneficial properties in treating some physical and psychological imbalances. Thus, we carried out a systematic review of studies published up to December 2022, where these themes...

Evaluation of the In Vitro Wound-Healing Potential of Ayahuasca.

Molecules (Basel, Switzerland) September 6, 2022 Joana Gonçalves, Ângelo Luís, Eugenia Gallardo et al. 5 citations

Ayahuasca is an Amazonian drink, which contains β-carboline alkaloids and N,N-dimethyltryptamine. The aim of this study was to evaluate the healing potential of decoctions of a commercial mixture, four individual plants and four mixtures of two plants used in the ayahuasca preparation. Thus, the cytotoxic potential of the samples was evaluated and a wound-healing assay was performed with a NHDF...

In Vitro Study of the Bioavailability and Bioaccessibility of the Main Compounds Present in Ayahuasca Beverages

Molecules September 13, 2021 Joana Gonçalves, Miguel Castilho, Tiago Rosado et al. 9 citations

Ayahuasca is a psychoactive beverage that contains the psychoactive compound N,N-dimethyltryptamine and β-carboline alkaloids. This study aims at determining in vitro the bioavailability and bioaccessibility of the main compounds present in decoctions of four individual plants, in a commercial mixture and in four mixtures of two individual plants used in the preparation of Ayahuasca. The...

Psychoactive Substances of Natural Origin: Toxicological Aspects, Therapeutic Properties and Analysis in Biological Samples.

Molecules (Basel, Switzerland) March 5, 2021 Joana Gonçalves, Ângelo Luís, Eugenia Gallardo et al. 39 citations

The consumption of new psychoactive substances (NPSs) has been increasing, and this problem affects several countries worldwide. There is a class of NPSs of natural origin, consisting of plants and fungi, which have a wide range of alkaloids, responsible for causing relaxing, stimulating or hallucinogenic effects. The consumption of some of these substances is prompted by religious beliefs and...

Evaluation of the Cytotoxicity of Ayahuasca Beverages

Molecules November 28, 2020 Ana Y. Simão, Joana Gonçalves, Ana Gradillas et al. 19 citations

Ayahuasca is a beverage consumed at shamanic ceremonies and currently has gained popularity on recreational scenarios. It contains beta-carboline alkaloids and N,N-dimethyltryptamine, which possesses hallucinogenic effects. Only a few studies have elicited the psychoactive effects and the dose of such compounds on neurological dopaminergic cells or animals. In this work, we aimed to study the...

Ayahuasca Beverages: Phytochemical Analysis and Biological Properties

Antibiotics October 24, 2020 Joana Gonçalves, Ângelo Luís, Ana Gradillas et al. 25 citations

Ayahuasca is a psychoactive beverage, originally consumed by indigenous Amazon tribes, of which consumption has been increasing worldwide. The aim of this study was to evaluate the phytochemical profile, as well as the antioxidant, anti-inflammatory and antimicrobial properties of decoctions of four individual plants, a commercial mixture and four mixtures of two individual plants used in the...

Psilocybin as a New Approach to Treat Depression and Anxiety in the Context of Life-Threatening Diseases—A Systematic Review and Meta-Analysis of Clinical Trials

Biomedicines September 5, 2020 Ana Sofia Vargas, Ângelo Luís, Mário Barroso et al. 121 citations

Psilocybin is a naturally occurring tryptamine known for its psychedelic properties. Recent research indicates that psilocybin may constitute a valid approach to treat depression and anxiety associated to life-threatening diseases. The aim of this work was to perform a systematic review with meta-analysis of clinical trials to assess the therapeutic effects and safety of psilocybin on those...

Toxicological Aspects and Determination of the Main Components of Ayahuasca: A Critical Review

Medicines October 18, 2019 Ana Y. Simão, Joana Gonçalves, Ana Paula Duarte et al. 36 citations

Ayahuasca is a psychoactive beverage prepared traditionally from a mixture of the leaves and stems of Psychotria viridis and Banisteriopsis caapi, respectively, being originally consumed by indigenous Amazonian tribes for ritual and medicinal purposes. Over the years, its use has spread to other populations as a means to personal growth and spiritual connection. Also, the recreational use of...

A unique natural selective kappa-opioid receptor agonist, salvinorin A, and its roles in human therapeutics.

Phytochemistry May 1, 2017 André Cruz, Sara Domingos, Eugenia Gallardo et al. 44 citations

Until the mid-60s, only the Mazatecs, an indigenous group from Oaxaca, Mexico, used Salvia Divinorum (S. divinorum) due to its hallucinogen properties. Later it was found that the hallucinogen effects of this plant were caused by the presence of a neoclerodane diterpene Salvinorin A (salvinorin A), which is a highly selective agonist of kappa-opioid receptor (KOR) that cause more intense...

Salvia divinorum: toxicological aspects and analysis in human biological specimens.

Bioanalysis July 1, 2016 Cláudia Margalho, Francisco Corte Real, Manuel López-rivadulla et al.

The identification and quantitation of the main psychoactive component of Salvia divinorum (salvinorin A) in biological specimens are crucial in forensic and clinical toxicology. Despite all the efforts made, its uncontrolled abuse has increased quickly, exposing its users' health to serious risks both in the short and long term. The use of alternative biological matrices in toxicological...

Determination of "new psychoactive substances" in postmortem matrices using microwave derivatization and gas chromatography-mass spectrometry.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences May 1, 2016 Cláudia Margalho, Alice Castanheira, Francisco Corte Real et al. 33 citations

Despite worldwide efforts aiming to ban the marketing and subsequent abuse of psychoactive substances such as synthetic cathinones and phenethylamines, there has been an alarming growth of both in recent years. Different compounds similar to those already existing are continuously appearing in the market in order to circumvent the legislation. An analytical methodology has been validated for...

Cytotoxic Effects of Salvinorin A, A Major Constituent of Salvia divinorum.

Medicinal chemistry (Shariqah (United Arab Emirates)) 2016 Ana Martinho, Sara M Silva, Eugenia Gallardo

S. divinorum is a psychoactive plant that has been consumed as a recreational drug of abuse in the last years. Salvinorin A is its main constituent, and is responsible for the observed psychoactive effects. Both S. divinorum and salvinorin A have become controlled drugs in several countries, but they are not listed in the Schedules of the United Nations Drug Conventions. Regarding the effects...