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Martin Kuchař

University of Chemistry and Technology, Prague, National Institute of Mental Health

65 papers in the library · 1,442 citations · publishing 2015-2026

Papers

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Metabolic profile of 25E-NBOH in human liver microsomes, rat urine, and fungus Cunninghamella elegans.

Journal of Pharmaceutical and Biomedical Analysis August 1, 2026 Magdaléna Vágnerová, Petr Palivec, Monika Mrňavá et al.

N-benzylphenethylamines are a class of new psychoactive substances (NPS) that are increasingly being used as recreational drugs with a wide range of adverse effects, possibly even death. Currently, the appearance of new N-benzylphenethylamines far outweighs the studies of their metabolism. One of such compounds, 25E-NBOH, has been on the market for the last seven years but its pharmacological...

Perinatal LC-ω-3-PUFA supplementation offsets prenatal THC-induced cognitive deficit via sex-specific modulation of hippocampal neuroplasticity.

European Journal of Pharmacology June 1, 2026 Gianluca Lavanco, Valentina Castelli, Cesare D'Amico et al.

Long-chain polyunsaturated fatty acids (LC-PUFAs) are crucial for brain development, with ω-3 species supporting neuronal membrane architecture, synaptogenesis and cognitive maturation, and ω-6 species linking lipid metabolism to neuronal signalling via the endocannabinoid system (ECS), the network of receptors, lipid mediators, and enzymatic pathways that orchestrate neurodevelopmental...

Multidimensional analysis of social withdrawal in the sub-chronic phencyclidine rat model for schizophrenia.

Psychopharmacology April 23, 2026 Stefani Kalli, Alina Davletova, Lenka Seillier et al.

RATIONALE: Social withdrawal in the sub-chronic phencyclidine (PCP) rat model—a behavioral correlate of schizophrenia’s negative symptoms—is well characterized, yet the psychological mechanisms underlying this deficit remain unclear. OBJECTIVES: Despite the complexity of social behavior, assessments often rely on a single metric (e.g., total interaction time) that has limited explanatory power,...

Psilocybin and Ibogaine in Cocaine‐Seeking: Extinction Enhancement Without Relapse Prevention

Addiction Biology March 1, 2026 Isis Koutrouli, Vojtěch Brejtr, Marek Schwendt et al.

Psychedelics have emerged as potential therapeutics for substance use disorders, yet preclinical data validating their efficacy remain limited. Here, we investigated the effects of a clinically inspired dose-escalation protocol of psilocybin and ibogaine on extinction and cue-induced reinstatement in Wistar male rats following intravenous cocaine self-administration (IVSA). Rats were trained on...

Development and optimization of a reliable HPLC-MS method for the identification of Tabernanthe iboga and its differentiation from toxic look-alikes

Planta Medica March 1, 2026 K Knížková, D Lovás, R Batelková et al.

Tabernanthe iboga is a plant containing a broad spectrum of alkaloids, the most significant of which is ibogaine. This alkaloid has been proven effective in the treatment of addiction to various substances, as it alleviates withdrawal symptoms and reduces cravings. Due to these properties, ibogaine clinics have emerged worldwide, incorporating ibogaine into treatment programs. [ 1 ] [ 2 ]...

Inhibition of Fatty Acid Amide Hydrolase Alters Cortical CREB Signaling and Social Behavior in a Rat Model of Schizophrenia

European Journal of Neuroscience February 1, 2026 Aditya R. Kumar, Lenka Seillier, Martin Kuchař et al.

Social withdrawal is a core negative symptom of schizophrenia and remains poorly understood at the molecular level. Dysregulation of the endocannabinoid system has been implicated in social behavior, yet its downstream signaling mechanisms are not fully characterized. In this context, this study examined the effects of the fatty acid amide hydrolase (FAAH) inhibitor, URB597, on CREB expression...

The effects of psilocybin on time perception in humans: A comparative analysis of subjective and objective measures

Journal of Psychopharmacology 2026 Petr Scholle, Štěpán Wenke, Tereza Nekovářová et al. 1 citation

Background: Although psychedelics have regained attention as potential treatment tools for various mental disorders, little research has examined their impact on temporal perception. Aims: This double-blinded placebo-controlled study aimed to investigate changes in temporal perception under psilocybin, both through performance during the Temporal Bisection Task (TBT) and through subjective...

The crystal structures of methoxmetamine hydrochloride and methoxetamine hydrochloride determined from laboratory X-ray powder diffraction data contained in the Powder Diffraction File ™

Powder Diffraction December 1, 2025 Analio Dugarte-Dugarte, Jacco van de Streek, Graciela Dı́az de Delgado et al.

Abstract The crystal structures of two arylcyclohexylamine derivatives – methoxmetamine·HCl (2-(3-methoxyphenyl)-2-(methylamino)cyclohexan-1-one hydrochloride, MMXE·HCl) and methoxetamine·HCl (2-(ethylamino)-2-(3-methoxyphenyl)cyclohexan-1-one hydrochloride, MXE·HCl) – have been determined using laboratory X-ray powder diffraction data contained in the Powder Diffraction File ™ . MMXE·HCl and...

Microdosing psilocybin and its effect on creativity: Lessons learned from three double-blind placebo controlled longitudinal trials

Neuropharmacology November 2, 2025 Luisa Prochazkova, Josephine Marschall, Michiel van Elk et al. 2 citations

BackgroundTaking very small doses of psychedelics (LSD, truffles) over an extended period became prevalent in Western societies for its alleged cognitive benefit, including enhanced creativity. However, in the absence of robust, double-blind-controlled quantitative studies, such claims remain anecdotal.MethodsHere we present results from 3 double-blind placebo-controlled longitudinal trials...

Cognitive and subjective effects of psilocybin microdosing: Results from two double-blind placebo-controlled longitudinal trials

Neuropharmacology October 17, 2025 Luisa Prochazkova, Josephine Marschall, Dominique P. Lippelt et al. 3 citations

ObjectiveMicrodosing psychedelics has been widely reported to enhance focus and problem-solving, sparking interest in its potential to treat attentional disorders such as ADHD. However, existing studies largely rely on anecdotal evidence and lack adequate placebo control.MethodsThis study contributes to the literature by examining the longitudinal effects of microdosing psilocybin truffles in...

Cross-Species Evidence for Psilocin-Induced Visual Distortions: Apparent Motion Is Perceived by Both Humans and Rats.

Biological Psychiatry Global Open Science September 1, 2025 Čestmír Vejmola, Klára Šíchová, Kateřina Syrová et al. 4 citations

Psychedelics, particularly psilocin, are increasingly being studied for their mind-altering effects and potential therapeutic applications in psychiatry. Visual hallucinations, especially the illusion of motion in static images, are a hallmark of their action. Despite growing interest, the underlying mechanisms remain poorly understood, as their systematic evaluation in both humans and animals...

Hexahydrocannabinol: pharmacokinetics, systemic toxicity, and acute behavioral effects in Wistar rats.

The International Journal of Neuropsychopharmacology August 1, 2025 Klára Šíchová, Barbara Mallarino, Lucie Janeckova et al. 1 citation

Hexahydrocannabinol (HHC) is a new psychoactive substance known for its mind-altering effects and temporary legal status. It is widely used in parts of the Europe and United Kingdom as a legal alternative to ∆9-tetrahydrocannabinol, yet little research has explored its effects and safety. This study examined how HHC is processed in the body, its toxicity, and its impact on behavior in male...

Behavioural and pharmacological evaluation of the psilocybin analogue baeocystin in Wistar rats.

Progress in neuro-psychopharmacology & biological psychiatry July 5, 2025 Hynek Danda, Kristýna Mazochová, Klára Šíchová et al. 1 citation

Baeocystin is a naturally occurring tryptamine-based compound found in various psychoactive mushrooms, including in several species of Psilocybe genus. Due to its structural similarity to psilocybin, which has shown a therapeutic potential in treatment of psychiatric disorders, there is a growing interest in investigating whether baeocystin exhibits comparable effects. This study investigated...

The phenomenology of psilocybin's experience mediates subsequent persistent psychological effects independently of sex, previous experience, or setting.

Pharmacological reports : PR June 16, 2025 Tereza Klučková, Marek Nikolič, Filip Tylš et al. 4 citations

Recent studies intensively explore psilocybin's antidepressant potential, but variables like previous experience, repeated use, setting, and sex remain underexplored. This study examines acute and long-term effects of psilocybin in healthy individuals. A double-blind, placebo-controlled, cross-over study included 40 healthy participants (20 females, mean age 38). Each received two doses of...

Achiral LC-MS/MS and chiral SFC-MS methods for quantification of methoxphenidine and O-desmethyl-methoxphenidine metabolite in rat serum and brain.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences June 1, 2025 Natalie Paškanová, Magdaléna Vágnerová, Bronislav Jurásek et al. 1 citation

Methoxphenidine (MXP), a dissociative anaesthetic derivative, has garnered the attention of toxicologists for their increasing abuse and associated toxicity. Despite that there is limited forensic and clinical toxicology data on MXP, especially regarding its metabolism and enantiomers. To fill this gap, we developed, validated and applied achiral liquid chromatography-tandem mass spectrometry...

Effects of serotonergic psychedelics on synaptogenesis and immediate early genes expression - comparison with ketamine, fluoxetine and lithium.

Journal of psychopharmacology (Oxford, England) May 28, 2025 Yana Vella, Kateřina Syrová, Aneta Petrušková et al. 1 citation

Recent evidence suggests that psychedelics can induce rapid and long-lasting antidepressant effects. The generally acknowledged explanation for these traits is the phenomenon of neuroplasticity, although the exact underlying molecular mechanisms remain unclear. This study investigates the effects of psilocin, lysergic acid diethylamide (LSD) and N,N-dimethyltryptamine (DMT) on synaptogenesis...

Effects of Clozapine, Haloperidol, and the NMDA Antagonist Ketamine on Novel Object Recognition in Gnathonemus petersii: A New Possible Model for Schizophrenia Research

Fishes May 15, 2025 Petra Horká, Josefina Mavrogeni, Veronika Langová et al. 1 citation

In animal models, ketamine, a non-competitive N-methyl-D-aspartate (NMDA) antagonist, induces schizophrenia-like symptoms, such as positive and negative symptoms, as well as cognitive deficits. In the present study, we evaluated the behavioral responses and the number of EODs (electric organ discharges) of the weakly electric fish Gnathonemus petersii using the novel object recognition task...

Preliminary pharmacokinetic and psychophysical investigations after controlled oral and inhalative consumption of hexahydrocannabinol (HHC)

Scientific Reports March 24, 2025 Lisa Höfert, Benjamin Franz, Cedric Groß et al. 2 citations

The semi-synthetic cannabinoid hexahydrocannabinol (HHC) has become a highly discussed topic in forensic toxicology since 2022 due to its legal availability at this time and its psychoactive effects. This study aimed to investigate the pharmacokinetics, effects, and immunological detectability of HHC after oral (25 mg HHC fruit gum) and inhalative (three puffs from HHC vape) consumption with...

The acute effects of methoxphenidine on behaviour and pharmacokinetics profile in animal model.

Progress in neuro-psychopharmacology & biological psychiatry March 20, 2025 Kristýna Štefková-mazochová, Hynek Danda, Vladimír Mazoch et al. 3 citations

Methoxphenidine (MXP) is classified as a new psychoactive substance that has recently emerged on the illicit drug market. Understanding the pharmacological and behavioural profiles of newly emerging drugs is essential for a better understanding of their psychotropic effects and potential toxicity. Therefore, in this study, we investigated a broad range of effects of acute MXP administration:...

METHOXPHENIDINE, A DESIGNER DRUG OR A POSSIBLE ROUTE FOR RESEARCH TO DEPRESSION TREATMENT?

International Journal of Neuropsychopharmacology February 12, 2025 *Bronislav Jurásek, Magdaléna Vágnerová, Hana Walaská et al.

Abstract Background Methoxphenidine (MXP) is a new psychoactive substance that belongs to the group of arylethylamines. Even though MXP has a different pharmacophore than ketamine, both of these substances belong to the dissociatives. To date, MXP has been associated with several intoxications and despite it being banned in most countries it is still available over the internet.1 Aims &...

DETERMINATION OF TRYPTAMINE ALKALOIDS AND THEIR STABILITY IN PSYCHOTROPIC MUSHROOMS

The International Journal of Neuropsychopharmacology February 1, 2025 Martin Kuchař, Klara Gotwaldova, Jan Borovička et al. 1 citation

Abstract Synthetic psilocybin (PSB), but also the PSB-containing mushrooms are used for psychedelic therapy and microdosing. It is necessary to know alkaloids variability and stability in wild-grown mushrooms. Concentrations of other psychotropic (or potentially psychotropic) tryptamines such as psilocin (PS), baeocystin (BA), norbaeocystin (NB), and aeruginascin (AE) are also relevant. This...

Exploring 5-MeO-DMT as a pharmacological model for deconstructed consciousness.

Neuroscience of Consciousness 2025 Christopher Timmermann, James W. Sanders, David Reydellet et al. 19 citations

5-MeO-DMT is a short-acting psychedelic that is anecdotally reported to induce a radical disruption of the self and a paradoxical quality of aroused, waking awareness that is nevertheless devoid of any specific perceptual contents. Here, we conducted an exploratory observational study of the phenomenological and neuronal effects of this compound. We collected micro-phenomenological interviews,...

Prefrontal electrophysiological biomarkers and mechanism-based drug effects in a rat model of alcohol addiction.

Translational Psychiatry December 5, 2024 Bettina Habelt, Dzmitry Afanasenkau, Cindy Schwarz et al. 6 citations

Patients with alcohol use disorder (AUD) who seek treatment show highly variable outcomes. A precision medicine approach with biomarkers responsive to new treatments is warranted to overcome this limitation. Promising biomarkers relate to prefrontal control mechanisms that are severely disturbed in AUD. This results in reduced inhibitory control of compulsive behavior and, eventually, relapse....

Prenatal THC exposure drives sex-specific alterations in spatial memory and hippocampal excitatory/inhibitory balance in adolescent rats.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie December 1, 2024 Valentina Castelli, Gianluca Lavanco, Giuseppe Tringali et al.

The interaction between the main psychotropic ingredient of Cannabis, Δ⁹- tetrahydrocannabinol (THC), with the endogenous cannabinoid system (ECS) is a critical and underrated issue that deserves utmost attention. The ECS, indeed, contributes to the formation and regulation of excitatory and inhibitory (E/I) neuronal networks that in the hippocampus underly spatial memory. This study explored...

Psilocybin administered following extinction sessions does not affect subsequent cocaine cue reinstatement in male and female rats and mice.

Neuroscience November 1, 2024 Veronika Pohořalá, Martin Kuchař, Rainer Spanagel et al. 3 citations

There are currently no pharmacological treatments for cocaine use disorder. Recently there has been a great deal of interest in the potential of psychedelic drugs such as psilocybin to treat psychiatric disorders. Human studies have indicated that a single administration of psilocybin can have long-lasting effects. Few preclinical studies have examined a role for psilocybin in addiction models....