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Bronislav Jurásek

18 papers in the library · 158 citations · publishing 2015-2026

Papers

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Determination of Enantiomers of Deschloroketamine and Its Metabolites in Wistar Rat Brains

Journal of Separation Science September 1, 2026 Natalie Paškanová, Vendulka Hrubá, Peter Frühauf et al.

ABSTRACT Deschloroketamine (DXE) is a dissociative new psychoactive substance whose enantiomers and metabolites may exhibit distinct pharmacological and toxicological profiles. Reliable analytical methods enabling the chiral determination of DXE and its metabolites in biological matrices are thus essential. In this study, we evaluated supercritical fluid chromatography coupled to tandem mass...

Metabolic profile of 25E-NBOH in human liver microsomes, rat urine, and fungus Cunninghamella elegans.

Journal of Pharmaceutical and Biomedical Analysis August 1, 2026 Magdaléna Vágnerová, Petr Palivec, Monika Mrňavá et al.

N-benzylphenethylamines are a class of new psychoactive substances (NPS) that are increasingly being used as recreational drugs with a wide range of adverse effects, possibly even death. Currently, the appearance of new N-benzylphenethylamines far outweighs the studies of their metabolism. One of such compounds, 25E-NBOH, has been on the market for the last seven years but its pharmacological...

The crystal structures of methoxmetamine hydrochloride and methoxetamine hydrochloride determined from laboratory X-ray powder diffraction data contained in the Powder Diffraction File ™

Powder Diffraction December 1, 2025 Analio Dugarte-Dugarte, Jacco van de Streek, Graciela Dı́az de Delgado et al.

Abstract The crystal structures of two arylcyclohexylamine derivatives – methoxmetamine·HCl (2-(3-methoxyphenyl)-2-(methylamino)cyclohexan-1-one hydrochloride, MMXE·HCl) and methoxetamine·HCl (2-(ethylamino)-2-(3-methoxyphenyl)cyclohexan-1-one hydrochloride, MXE·HCl) – have been determined using laboratory X-ray powder diffraction data contained in the Powder Diffraction File ™ . MMXE·HCl and...

Behavioural and pharmacological evaluation of the psilocybin analogue baeocystin in Wistar rats.

Progress in neuro-psychopharmacology & biological psychiatry July 5, 2025 Hynek Danda, Kristýna Mazochová, Klára Šíchová et al. 1 citation

Baeocystin is a naturally occurring tryptamine-based compound found in various psychoactive mushrooms, including in several species of Psilocybe genus. Due to its structural similarity to psilocybin, which has shown a therapeutic potential in treatment of psychiatric disorders, there is a growing interest in investigating whether baeocystin exhibits comparable effects. This study investigated...

Achiral LC-MS/MS and chiral SFC-MS methods for quantification of methoxphenidine and O-desmethyl-methoxphenidine metabolite in rat serum and brain.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences June 1, 2025 Natalie Paškanová, Magdaléna Vágnerová, Bronislav Jurásek et al. 1 citation

Methoxphenidine (MXP), a dissociative anaesthetic derivative, has garnered the attention of toxicologists for their increasing abuse and associated toxicity. Despite that there is limited forensic and clinical toxicology data on MXP, especially regarding its metabolism and enantiomers. To fill this gap, we developed, validated and applied achiral liquid chromatography-tandem mass spectrometry...

Preliminary pharmacokinetic and psychophysical investigations after controlled oral and inhalative consumption of hexahydrocannabinol (HHC)

Scientific Reports March 24, 2025 Lisa Höfert, Benjamin Franz, Cedric Groß et al. 2 citations

The semi-synthetic cannabinoid hexahydrocannabinol (HHC) has become a highly discussed topic in forensic toxicology since 2022 due to its legal availability at this time and its psychoactive effects. This study aimed to investigate the pharmacokinetics, effects, and immunological detectability of HHC after oral (25 mg HHC fruit gum) and inhalative (three puffs from HHC vape) consumption with...

The acute effects of methoxphenidine on behaviour and pharmacokinetics profile in animal model.

Progress in neuro-psychopharmacology & biological psychiatry March 20, 2025 Kristýna Štefková-mazochová, Hynek Danda, Vladimír Mazoch et al. 3 citations

Methoxphenidine (MXP) is classified as a new psychoactive substance that has recently emerged on the illicit drug market. Understanding the pharmacological and behavioural profiles of newly emerging drugs is essential for a better understanding of their psychotropic effects and potential toxicity. Therefore, in this study, we investigated a broad range of effects of acute MXP administration:...

Intriguing Cytotoxicity of the Street Dissociative Anesthetic Methoxphenidine: Unexpected Impurities Spotted

International Journal of Molecular Sciences February 14, 2022 Bronislav Jurásek, Silvie Rimpelová, Martin Babor et al. 7 citations

The black market for new psychoactive substances has been constantly evolving and the substances that appear on this market cause a considerable number of issues, in extreme cases leading to human deaths. While monitoring the drug black market, we detected a sample of a dissociative anesthetic methoxphenidine, the salt of which contained an unusual anion in the form of bromo- and chloro-zincate...

Pharmacokinetic, pharmacodynamic, and behavioural studies of deschloroketamine in Wistar rats.

British Journal of Pharmacology 2022 Kristýna Štefková-mazochová, Hynek Danda, Wim Dehaen et al. 13 citations

Deschloroketamine (DCK), a structural analogue of ketamine, has recently emerged on the illicit drug market as a recreational drug with a modestly long duration of action. Despite it being widely used by recreational users, no systematic research on its effects has been performed to date. Pharmacokinetics, acute effects, and addictive potential in a series of behavioural tests in Wistar rats...

2C-B-Fly-NBOMe Metabolites in Rat Urine, Human Liver Microsomes and C. elegans: Confirmation with Synthesized Analytical Standards.

Metabolites November 12, 2021 Jitka Nykodemová, Anna Šuláková, Petr Palivec et al. 14 citations

Compounds from the N-benzylphenethylamine (NBPEA) class of novel psychoactive substances are being increasingly utilized in neurobiological and clinical research, as diagnostic tools, or for recreational purposes. To understand the pharmacology, safety, or potential toxicity of these substances, elucidating their metabolic fate is therefore of the utmost interest. Several studies on NBPEA...

Synthesis and identification of deschloroketamine metabolites in rats' urine and a quantification method for deschloroketamine and metabolites in rats' serum and brain tissue using liquid chromatography tandem mass spectrometry

Drug Testing and Analysis October 31, 2019 Kateřina Hájková, Bronislav Jurásek, Jan Čejka et al. 12 citations

Deschloroketamine (2-(methylamino)-2-phenyl-cyclohexanone) is a ketamine analog belonging to a group of dissociative anesthetics, which have been distributed within the illicit market since 2015. However, it was also being sold as 'ketamine' misleading people to believe that they were getting genuine ketamine. Dissociative anesthetics have also come to the attention of the psychiatric field due...

Synthesis, absolute configuration and in vitro cytotoxicity of deschloroketamine enantiomers: rediscovered and abused dissociative anaesthetic

New Journal of Chemistry 2018 Bronislav Jurásek, František Králík, Silvie Rimpelová et al. 15 citations

Deschloroketamine has been synthesized, the absolute configuration of enantiomers elucidated and the in vitro cytotoxicity of the enantiomers determined using nine different cell lines.

X-ray powder diffraction data for methoxetamine hydrochloride, C 15 H 22 ClNO 2

Powder Diffraction August 22, 2017 J. Maixner, Bronislav Jurásek, Michal Himl et al. 2 citations

X-ray powder diffraction data, unit-cell parameters and space group for 2-(ethylamino)-2-(3-methoxyphenyl)cyclohexan-1-one hydrochloride, C 15 H 22 ClNO 2 , are reported [ a = 8.574(2) Å, b = 9.943(2) Å, c = 8.774(1) Å, β = 100.294(3)°, unit-cell volume V = 736(1) Å 3 , Z = 2, and space-group P 2 1 ]. All measured lines were indexed and are consistent with the P 2 1 space group. No detectable...

X-ray powder diffraction data for ( S )-Deschloroketamine hydrochloride, C 13 H 18 ClNO

Powder Diffraction June 21, 2017 J. Maixner, Bronislav Jurásek, Michal Kohout et al. 5 citations

X-ray powder diffraction data, unit-cell parameters, and space group for S -2-(methylamino)-2-phenylcyclohexan-1-one hydrochloride, C 13 H 18 ClNO, are reported [ a = 6.578 (1) Å, b = 13.250 (2) Å, c = 15.096 (2) Å, β = 91.619 (3)°, unit-cell volume V = 1315(1) Å 3 , Z = 4, and space group P 2 1 ]. All measured lines were indexed and are consistent with the P 2 1 space group. No detectable...

Synthesis of methoxetamine, its metabolites and deuterium labelled analog as analytical standards and their HPLC and chiral capillary electrophoresis separation

RSC Advances 2017 Bronislav Jurásek, Michal Himl, Radek Jurok et al. 13 citations

Methoxetamine, a designer drug marketed as a replacement for the dissociative anaesthetic ketamine, has been associated with significant numbers of hospital related intoxications and deaths in Europe.

Detailed pharmacological evaluation of methoxetamine (MXE), a novel psychoactive ketamine analogue—Behavioural, pharmacokinetic and metabolic studies in the Wistar rat

Brain Research Bulletin May 4, 2016 Rachel R. Horsley, Eva Lhotková, Kateřina Hájková et al. 38 citations

Methoxetamine (MXE) is a novel psychoactive compound (NPS) that emerged in 2010 as a substitute for the dissociative anaesthetic ketamine. MXE has a reputation of carrying a lower risk of harm than ketamine, however a number of deaths have been reported. Currently very little is known about the psychopharmacological effects of this compound or its toxicity; therefore we tested, in Wistar rats,...

Salting-out-assisted liquid–liquid extraction as a suitable approach for determination of methoxetamine in large sets of tissue samples

Analytical and Bioanalytical Chemistry December 11, 2015 Kateřina Hájková, Bronislav Jurásek, David Sýkora et al. 26 citations

A new designer drug, a dissociative anesthetic, and a putative N-methyl-D-aspartate receptor antagonist, methoxetamine (MXE) noted by the EU Early Warning System has been already identified as a cause of several fatalities worldwide. The primary objective of this work was to develop a suitable sample preparation method allowing for isolation of MXE and its main metabolites in high yields from...