Abstract A method has been developed for the rapid isolation and identification of psilocin from psilocin/psilocybin-containing mushrooms. Based on the difference in the solubility properties in butyl chloride of psilocin and other constituents present in psilocin/psilocybin-containing mushrooms, psilocin was easily separated in pure form.
Abstract A simple aqueous extraction method for the isolation and identification of psilocin from Psilocybe cubensis mushrooms is reported. This method employs a dephosphorylation of the phosphate ester to psilocin, which facilitates a greater product yield and simplifies identification. Psilocin extracted by this method is sufficiently concentrated and free of cocontaminants to allow...
A screening of Norwegian mushrooms for the presence of hallucinogenic indole alkaloids was carried out using HPLC with ultraviolet, fluorescence and electrochemical detection. The analysis revealed psilocybin and psilocin in two mushroom species: PLUTEUS SALICINUS and CONOCYBE CYANOPUS. The content of psilocybin and psilocin in dried material of PLUTEUS SALICINUS was determined to be 0.35% and...
The use of a hallucinogenic mushroom, PSILOCYBE SEMILANCEATA, has been occasionally reported in Finland, where the species is widely distributed. We have determined, by HPLC, the content of psilocybin and psilocin in P. SEMILANCEATA samples collected from different parts of Finland; the psilocybin content was found to be high (0.62-2.37%, mean 1.42% of dry weight), some samples also contained...
Abstract A method using high performance liquid chromatography (HPLC) with an acetonitrile, water, and phosphoric acid mobile phase and a bonded cyano-amino-type polar phase column has been developed for the rapid, selective, and accurate quantitation of psilocybin in dry mushroom material. A simple one-step procedure is used for the quantitative extraction of psilocybin in under 60 min. The...
1 We have reviewed the clinical features and management of 44 consecutive patients presenting to hospital over a 5 week period during an outbreak of ingestion of psilocybin containing mushrooms. 2 Patients presented to hospital usually because of dysphoric effects an average of 3.8 h after ingesting the mushrooms. 3 Mydriasis was present in 40 patients but fewer than half showed other...
The analysis of twenty species from seven genera of Pacific Northwest mushrooms revealed psilocybin (and in some cases psilocin as well) in seven species from three genera. The species found to contain psilocybin (and psilocin) varied from one collection to another by more than a factor of seven in amount present. Total psilocybin and psilocin levels in species known to be in use for...
Analysis of Psilocybe cubensis (Earle) Singer grown in controlled culture showed that the level of psilocin was generally zero in the first (or sometimes even the second) fruiting of the mushroom from a given culture and that the level reached a maximum by the fourth flush. The level of psilocybin, which was nearly always at least twice the level of psilocin, showed no upward or downward trend...
Fifteen rats were trained to discriminate between the tryptamine hallucinogen psilocybin (4-phosphoryloxy-N,N-dimethyltryptamine; 1.0 mg/kg) and saline in a two-lever choice task. Dose-response and time-response curves were obtained. The psilocybin cue generalized to psilocin (the dephosphorylated congener of psilocybin) and to the prototypical indoleamine hallucinogen LSD, but not to the...