A convenient analytical method for ibotenic acid (IBO) and muscimol (MUS) in a toxic mushroom, Amanita muscaria (A. muscaria), was developed. IBO and MUS in the mushroom were extracted with 70% methanol. After filtration, IBO and MUS in the extract were determined by high performance liquid chromatography (HPLC) with a UV detector set at 210nm. The HPLC system adopted was ion-pair...
Ibotenic acid (IBO) and muscimol (MUS) in the fruit body of Amanita muscaria during the reproduction stage were investigated. The mean levels of IBO and MUS throughout the fruit were x: 343ppm and x: 22ppm, respectively; most was detected in the cap of the fruit (IBO x: 519ppm, MUS x: 30ppm), then in the base (IBO x: 290ppm, MUS x: 20ppm), with the smallest amount in the stalk (IBO x: 253ppm,...
It is believed by people in mountainous areas that toxins in A. muscaria are reduced by drying, storing or cooking. The main physiologically active substances, ibotenic acid (IBO) and muscimol (MUS), in A. muscaria were therefore investigated. IBO is readily transformed to into MUS through decarboxylation, and MUS is a hallucinogen. Drying A. muscaria in the sun or with a heater caused an...