Simultaneous Analysis of Ibotenic Acid and Muscimol in Toxic Mushroom, Amanita muscaria, and Analytical Survey on Edible Mushrooms
Koujun Tsunoda, Noriko Inoue, Yasuo Aoyagi, Tatsuyuki Sugahara
Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 1993 DOI: 10.3358/shokueishi.34.12 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Analytical method development study Peer reviewed |
|---|---|
| Population | Amanita muscaria mushrooms and commercial edible mushrooms |
| Citations | 26 |
| Key points | The described HPLC method recovered more than 98% of added ibotenic acid and muscimol and detected each at a minimum of about 1 ppm. Amanita muscaria contained ibotenic acid at 258 to 471 ppm and muscimol at 18 to 27 ppm, while neither compound was detected in commercial edible mushrooms. |
Abstract
A convenient analytical method for ibotenic acid (IBO) and muscimol (MUS) in a toxic mushroom, Amanita muscaria (A. muscaria), was developed. IBO and MUS in the mushroom were extracted with 70% methanol. After filtration, IBO and MUS in the extract were determined by high performance liquid chromatography (HPLC) with a UV detector set at 210nm. The HPLC system adopted was ion-pair chromatography in the reverse-phase mode on an IRICA RP-18 (C18) column (4.0mm×25cm) with sodium dodecyl sulfate as a counter ion. Recoveries of IBO and MUS added to the sample were more than 98%, and the minimum detectable concentration of IBO or MUS was about 1ppm. The concentrations of IBO and MUS in A. muscaria ranged from 258 to 471ppm and from 18 to 27ppm, respectively. Neither of the compounds was detected in commercial edible mushrooms.
Citations in the library
Cited by 11
- Fungal Hallucinogens Psilocin, Ibotenic Acid, and Muscimol Therapeutic Drug Monitoring July 12, 2013
- Ibotenic Acid Biosynthesis in the Fly Agaric Is Initiated by Glutamate Hydroxylation. Angewandte Chemie International Edition July 20, 2020
- Analysis of hallucinogenic constituents in Amanita mushrooms circulated in Japan. Forensic Science International December 20, 2006
- Classics in Chemical Neuroscience: Muscimol. ACS Chemical Neuroscience September 18, 2024
- Quantitative LC-QToF-MS Analysis of Mycochemicals in Amanita muscaria, Psilocybe spp. (Agaricomycetes), and Consumer Products. International journal of medicinal mushrooms 2025
and 6 more in the library