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bioRxiv : the preprint server for biology

ISSN 2692-8205

45 papers in the library · 40 citations · publishing 2023-2026

Papers

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Global excitatory synchrony: Ketamine induces global common-mode excitatory network oscillation by decoupling key interneurons.

bioRxiv : the preprint server for biology August 2, 2025 Belen Karakullukcu, Hamilton White, Christopher Connor et al. preprint

Ketamine is a dissociative anesthetic used in subanesthetic doses with analgesic and anti-depressive properties. However, its mechanistic effects on neuronal signaling and circuit function remain underexplored. We address this shortcoming by employing multi-neuronal imaging in the simple nematode C. elegans that allows measurement of neuron activity across the animal's entire head with...

Psychedelic 5-HT2A agonist increases spontaneous and evoked 5-Hz oscillations in visual and retrosplenial cortex.

bioRxiv : the preprint server for biology July 6, 2025 Callum M. White, Zohre Azimi, Robert Staadt et al. 1 citation preprint

Visual perception appears largely stable in time. However, psychophysical studies have revealed that theta waves (4-8 Hz) can modulate perception and have been linked to various cognitive states and functions. Neither the contribution of theta waves to cortical activity nor their impact during aberrant brain states have been resolved at high spatiotemporal scales. Here, using cortex-wide...

Concurrent maternal stress and THC exposure during pregnancy alters adolescent behavioral outcomes and corticolimbic molecular programs.

bioRxiv : the preprint server for biology June 28, 2025 Jimmy Olusakin, Mahima Dewan, Atul Kashyap et al. preprint

Cannabis use during pregnancy is increasing, often to alleviate stress and anxiety, yet the long-term effect of prenatal cannabis exposure alone or in combination with psychosocial stress on offspring neurodevelopment or maternal behaviors remains unclear. Here, we developed a translational rodent model combining prenatal Δ9-tetrahydrocannabinol (THC) exposure with chronic psychosocial stress...

LSD Restores Synaptic Plasticity in VTA of Morphine-Treated Mice and Disrupts Morphine-Conditioned Place Preference.

bioRxiv : the preprint server for biology June 15, 2025 Michael Von Gunten, Tenna Russell, Isaac Stirland et al. preprint

Psychedelics are emerging as a promising treatment option for a range of neuropsychiatric disorders, including substance use disorders. One potential mechanism underlying their therapeutic benefits may involve a reversal of maladaptive plasticity induced by drug exposure. Here, we identify physiological, behavioral, and epigenetic impacts of lysergic acid diethylamide (LSD) on morphine-treated...

Behavioral and genetic analysis of the effects of the psychedelic 2,5-dimethoxy-4-iodoamphetamine (DOI) in C. elegans.

bioRxiv : the preprint server for biology May 23, 2025 Amanda M. White, Adele D. Bauer, Serge Faumont et al. preprint

Psychedelics show promise in treating depression, PTSD, and substance use disorder, prompting research into their mechanisms of action. Most studies use rodent models, but genetic tools can be challenging to apply. Invertebrate models, like C. elegans , offer a cost-effective alternative with short generation times and genetic tractability. This study examined the worm's response to the...

Learning and language in the unconscious human hippocampus.

bioRxiv : the preprint server for biology April 9, 2025 Kalman A Katlowitz, Shraddha Shah, Melissa C Franch et al. 11 citations preprint

Consciousness is a fundamental component of cognition, 1 but the degree to which higher-order perception relies on it remains disputed. 2,3 Here we demonstrate the persistence of learning, semantic processing, and online prediction in individuals under general anesthesia-induced loss of consciousness. 4,5 Using high-density Neuropixels microelectrodes 6 to record neural activity in the human...

Sub-Second Fluctuation between Top-Down and Bottom-Up Modes Distinguishes Diverse Human Brain States.

bioRxiv : the preprint server for biology March 28, 2025 Youngjai Park, Younghwa Cha, Hyoungkyu Kim et al. preprint

Information continuously flows between regions of the human brain, exhibiting distinct patterns that dynamically shift across states of consciousness, cognitive modes, and neuropsychiatric conditions. In this study, we introduce Relative Phase Analysis (RPA), a method that leverages phase-lead/lag relationships to reveal the real-time dynamics of dominant directional patterns and their rapid...

Phenethylaminylation: Preliminary In Vitro Evidence for the Covalent Transamidation of Psychedelic Phenethylamines to Glial Proteins using 3,5-Dimethoxy-4-(2-Propynyloxy)-Phenethylamine as a Model Compound.

bioRxiv : the preprint server for biology February 17, 2025 Rajiv S. Rangan, R Max Petty, Suchismita Acharya et al. preprint

Psychedelics are well known for their ability to produce profoundly altered states of consciousness. But, more importantly, the effects of psychedelics can influence neurobehavioral changes that last well after these acute subjective effects end. This phenomenon is currently being leveraged in the development of psychedelic-assisted psychotherapies for the treatment of multiple neuropsychiatric...

Persistent large-scale changes in alternative splicing in prefrontal cortical neuron types following psychedelic exposure.

bioRxiv : the preprint server for biology January 16, 2025 Yungyi Hsiao, Marcos As Fonseca, Alina S Tiemroth et al. preprint

Psychedelics engage the serotonergic system as potent neuromodulators, increasing neuroplasticity in humans and rodents. Persistent changes in cognitive flexibility, emotional regulation, and social cognition are thought to underlie the therapeutic effects of psychedelics. However, the underlying molecular and cellular basis of psychedelic-induced plasticity remains unclear. Here, we identify...

Ciliopathy interacts with neonatal anesthesia to cause non-apoptotic caspase-mediated motor deficits.

bioRxiv : the preprint server for biology November 27, 2024 Nemanja Sarić, Zeynep Atak, Courtni Foster Sade et al. preprint

Increasing evidence suggests that anesthesia may induce developmental neurotoxicity, yet the influence of genetic predispositions associated with congenital anomalies on this toxicity remains largely unknown. Children with congenital heart disease often exhibit mutations in cilia-related genes and ciliary dysfunction, requiring sedation for their catheter or surgical interventions during the...

Neural Correlates of Psychedelic, Sleep, and Sedated States Support Global Theories of Consciousness.

bioRxiv : the preprint server for biology October 23, 2024 Rui Dai, Hyunwoo Jang, Anthony G. Hudetz et al. 1 citation preprint

Understanding neural mechanisms of consciousness remains a challenging question in neuroscience. A central debate in the field concerns whether consciousness arises from global interactions that involve multiple brain regions or focal neural activity, such as in sensory cortex. Additionally, global theories diverge between the Global Neuronal Workspace (GNW) hypothesis, which emphasizes frontal...

5-HT2C receptors in the nucleus accumbens constrain the rewarding effects of MDMA.

bioRxiv : the preprint server for biology October 22, 2024 Matthew B Pomrenze, Sam Vaillancourt, Juliana S Salgado et al. 1 citation preprint

MDMA is a promising adjunct to psychotherapy and has well-known abuse liability, although less than other amphetamine analogs. While the reinforcing dopamine (DA)-releasing properties of MDMA are on par with methamphetamine (METH), MDMA is a far more potent serotonin (5-HT) releaser, via the 5-HT transporter (SERT). MDMA-mediated 5-HT release in a major reward center, the nucleus accumbens...

Snapshot of 5-HT 2A receptor activation in the mouse brain via IP 1 detection.

bioRxiv : the preprint server for biology October 12, 2024 Mario de la Fuente Revenga, Javier González-Maeso preprint

The distinct subjective effects that define psychedelics such as LSD, psilocybin or DOI as drug class are causally linked to activation of the serotonin 2A receptor (5-HT 2A R). However, some aspects of 5-HT 2A R pharmacology remain elusive, such as what molecular drivers differentiate psychedelic from non-psychedelic 5-HT 2A R agonists. We developed an ex vivo platform to obtain snapshots of...

Mindfulness-based Neurofeedback: A Systematic Review of EEG and fMRI studies.

bioRxiv : the preprint server for biology September 15, 2024 Isaac N Treves, Keara D Greene, Zia Bajwa et al. 7 citations preprint

Neurofeedback concurrent with mindfulness meditation may reveal meditation effects on the brain and facilitate improved mental health outcomes. Here, we systematically reviewed EEG and fMRI studies of mindfulness meditation with neurofeedback (mbNF) and followed PRISMA guidelines. We identified 10 fMRI reports, consisting of 177 unique participants, and 9 EEG reports, consisting of 242...

Ketamine reverses stress-induced hypersensitivity to sunk costs.

bioRxiv : the preprint server for biology May 14, 2024 Romain Durand-De Cuttoli, Brian M Sweis 1 citation preprint

How mood interacts with information processing in the brain is thought to mediate the maladaptive behaviors observed in depressed individuals. However, the neural mechanisms underlying impairments in emotion-cognition interactions are poorly understood. This includes influencing the balance between how past-sensitive vs. future-looking one is during decision-making. Recent insights from the...

Bioisosteric analogs of MDMA with improved pharmacological profile.

bioRxiv : the preprint server for biology April 11, 2024 Ana Sofia Alberto-Silva, Selina Hemmer, Hailey A. Bock et al. preprint

3,4-Methylenedioxymethamphetamine (MDMA, ' ecstasy' ) is re-emerging in clinical settings as a candidate for the treatment of specific psychiatric disorders (e.g. post-traumatic stress disorder) in combination with psychotherapy. MDMA is a psychoactive drug, typically regarded as an empathogen or entactogen, which leads to transporter-mediated monoamine release. Despite its therapeutic...

Opioid receptor expressing neurons of the central amygdala gate behavioral effects of ketamine in mice.

bioRxiv : the preprint server for biology March 6, 2024 Matthew B Pomrenze, Sam Vaillancourt, Pierre Llorach et al. 1 citation preprint

Ketamine has anesthetic, analgesic, and antidepressant properties which may involve multiple neuromodulatory systems. In humans, the opioid receptor (OR) antagonist naltrexone blocks the antidepressant effect of ketamine. It is unclear whether naltrexone blocks a direct effect of ketamine at ORs, or whether normal functioning of the OR system is required to realize the full antidepressant...

A choroid plexus apocrine secretion mechanism shapes CSF proteome and embryonic brain development.

bioRxiv : the preprint server for biology January 16, 2024 Ya'El Courtney, Joshua P Head, Elizabeth D Yimer et al. 9 citations preprint

We discovered that apocrine secretion by embryonic choroid plexus (ChP) epithelial cells contributes to the cerebrospinal fluid (CSF) proteome and influences brain development in mice. The apocrine response relies on sustained intracellular calcium signaling and calpain-mediated cytoskeletal remodeling. It rapidly alters the embryonic CSF proteome, activating neural progenitors lining the...

Criticality of resting-state EEG predicts perturbational complexity and level of consciousness during anesthesia.

bioRxiv : the preprint server for biology October 31, 2023 Charlotte Maschke, Jordan O'Byrne, Michele Angelo Colombo et al. 7 citations preprint

Consciousness has been proposed to be supported by electrophysiological patterns poised at criticality, a dynamical regime which exhibits adaptive computational properties, maximally complex patterns and divergent sensitivity to perturbation. Here, we investigated dynamical properties of the resting-state electroencephalogram of healthy subjects undergoing general anesthesia with propofol,...

Paradoxical increases in anterior cingulate cortex activity during nitrous oxide-induced analgesia reveal a signature of pain affect.

bioRxiv : the preprint server for biology April 3, 2023 Jarret Ap Weinrich, Cindy D Liu, Madison E Jewell et al. preprint

The general consensus is that increases in neuronal activity in the anterior cingulate cortex (ACC) contribute to pain's negative affect. Here, using in vivo imaging of neuronal calcium dynamics in mice, we report that nitrous oxide, a general anesthetic that reduces pain affect, paradoxically, increases ACC spontaneous activity. As expected, a noxious stimulus also increased ACC activity....