Ibotenic Acid Biosynthesis in the Fly Agaric Is Initiated by Glutamate Hydroxylation.
Sebastian Obermaier, Michael Müller
Angewandte Chemie (International ed. in English) July 20, 2020 DOI: 10.1002/anie.202001870 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Experimental study Peer reviewed |
|---|---|
| Keywords | Biosynthesis Enzyme catalysis Fly agaric Hydroxylation Ibotenic acid |
| Citations | 59 |
| Key findings | A glutamate hydroxylase from Amanita muscaria, along with six surrounding biosynthetic genes, forms the genetic basis for ibotenic acid production. |
Abstract
The fly agaric, Amanita muscaria, is widely known for its content of the psychoactive metabolites ibotenic acid and muscimol. However, their biosynthetic pathway and the respective enzymes are entirely unknown. 50 years ago, the biosynthesis was hypothesized to start with 3-hydroxyglutamate. Here, we build on this hypothesis by the identification and recombinant production of a glutamate hydroxylase from A. muscaria. The hydroxylase gene is surrounded by six further biosynthetic genes, which we link to the production of ibotenic acid and muscimol using recent genomic and transcriptomic data. Our results pinpoint the genetic basis for ibotenic acid formation and thus provide new insights into a decades-old question concerning a centuries-old drug.