Determination of muscimol and ibotenic acid in Amanita mushrooms by high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry.
Kenji Tsujikawa, Kenji Kuwayama, Hajime Miyaguchi, Tatsuyuki Kanamori, Yuko T Iwata, Hiroyuki Inoue, Takemi Yoshida, Tohru Kishi
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences June 1, 2007 DOI: 10.1016/j.jchromb.2007.01.046 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Peer reviewed |
|---|---|
| Population | Amanita mushrooms naturally grown and obtained from the drug market |
| Key points | The described method quantifies muscimol and ibotenic acid in Amanita mushrooms, with linear calibration from 25 to 2500 ppm for muscimol and 40 to 2500 ppm for ibotenic acid, and was successfully applied to natural and market mushrooms. |
Abstract
A reliable analytical method was developed for the quantification and identification of muscimol (MUS) and ibotenic acid (IBO), the toxic constituents of Amanita muscaria and Amanita pantherina. MUS and IBO were extracted from mushrooms by aqueous methanol and derivatized with dansyl chloride (DNS-Cl). After extraction with ethyl acetate and evaporation of the solvent, the residue was ethylated with 1.25 M hydrogen chloride in ethanol. The resulting derivatives were quantified by high-performance liquid chromatography with UV detection and identified by liquid chromatography electrospray ionization tandem mass spectrometry. Calibration curves were linear in the range of 25-2500 ppm for MUS and 40-2500 ppm for IBO, respectively. This method was successfully applied to identify and quantify MUS and IBO in Amanita mushrooms naturally grown and circulated in the drug market.
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Cites 6
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- Hallucinogens and dissociative agents naturally growing in the United States. Pharmacology & Therapeutics May 1, 2004
- Fly agaric (Amanita muscaria) poisoning, case report and review. Toxicon June 1, 2005
- Analysis of hallucinogenic constituents in Amanita mushrooms circulated in Japan. Forensic Science International December 20, 2006
- Change in Ibotenic Acid and Muscimol Contents in Amanita muscaria during Drying, Storing or Cooking Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 1993
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