A systematic study of changes in monoamine neurotransmitters in the rat brain following acute administration of alpha-methyltryptamine (AMT), 5-methoxy-alpha-methyltryptamine (5-MeO-AMT) and 5-methoxy-N,N-diisopropyltryptamine (5-MeO-DiPT).
Kaixi Li, Nan Li, Yuanyuan Chen, Xiangyu Li, Yanling Qiao, Dan Wang, Bin Di, Peng Xu
Neuroscience Research July 1, 2025 DOI: 10.1016/j.neures.2025.04.006 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Experimental study Peer reviewed |
|---|---|
| Population | Rat brains |
| Interventions | AMT 5-MeO-AMT 5-MeO-DiPT |
| Topics | 5-MeO-DMT |
| Keywords | 5-MEO-Amt 5-MEO-Dipt Hplc-ecd Monoaminergic Neurotransmitters neurotransmitter systems Brain chemicals Neural signaling Tryptamines synthetic tryptamines |
| Citations | 1 |
| Key findings | AMT, 5-MeO-AMT, and 5-MeO-DiPT significantly affect monoaminergic neurotransmitters in rat brains, with effects differing across brain regions. |
Abstract
Alpha-methyltryptamine (AMT), 5-methoxy-alpha-methyltryptamine (5-MeO-AMT), and 5-methoxy-N,N-diisopropyltryptamine (5-MeO-DiPT) are three synthetic tryptamines with hallucinogenic properties that are widely abused worldwide. The hallucinogenic effects of tryptamines are primarily related to activation of the 5-HT receptor, and among the many subtypes of 5-HT receptors, the 5-HT2A receptor is the key receptor for hallucinogenic effects. In the present study, the monoamine neurotransmitters DA and its metabolites 3,4-Dihydroxyphenylacetic Acid (DOPAC) and homovanillic acid (HVA), 5-HT and its metabolite 5-hydroxyindoleacetic acid (5-HIAA) were systematically investigated in the prefrontal cortex (PFC), nucleus accumbent (NAc), dorsolateral striatum (DLS) and hippocampus (HIP) using a validated HPLC-ECD analytical method after administration of the three tryptamines at different doses. The results showed that the three tryptamines had certain effects and the effects were different in different brain regions and showed that AMT, 5-MeO-AMT and 5-MeO-DiPT had significant effects on monoaminergic neurotransmitters in rat brains. Among them, DAergic and serotonergic play important roles, and this study provides valuable information for further research on the neurochemical effects of tryptamine hallucinogens in the brain.
Comparable studies
Other experimental studies on 5-MeO-DMT, most cited first.
| Study | Year | Design | Participants |
|---|---|---|---|
| Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT. Human cerebral organoids | 2017 | Experimental study | |
| Bufo alvarius: a potent hallucinogen of animal origin. Bufo alvarius toad venom | 1994 | Experimental study | |
| Prospective examination of synthetic 5-methoxy-N,N-dimethyltryptamine inhalation: effects on salivary IL-6, cortisol levels, affect, and non-judgment Volunteers | 2019 | Within-subjects experimental study | n = 11 |
| Unmasking of a neonatal somatovesical reflex in adult cats by the serotonin autoreceptor agonist 5-methoxy-N,N-dimethyltryptamine. Adult cats | 1990 | Experimental study | n = 10 |
| Studies on the Mechanism of Action of Monoamine Oxidase: Metabolism of N,N-Dimethyltryptamine and N,N-Dimethyltryptamine-N-Oxide | 1962 | Experimental study |
Citations in the library
Cites 17
- Receptor interaction profiles of novel psychoactive tryptamines compared with classic hallucinogens. European Neuropsychopharmacology May 20, 2016
- The hallucinogenic world of tryptamines: an updated review. Archives of Toxicology August 1, 2015
- Recreational Use, Analysis and Toxicity of Tryptamines Current Neuropharmacology December 10, 2014
- Psychedelic-inspired drug discovery using an engineered biosensor. Cell April 28, 2021
- Hallucinogen-like actions of 5-methoxy-N,N-diisopropyltryptamine in mice and rats. Pharmacology, Biochemistry, and Behavior 2006
and 12 more in the library