Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT.
Vanja Dakic, Juliana Minardi Nascimento, Rafaela Costa Sartore, Renata de Moraes Maciel, Draulio B. de Araujo, Sidarta Ribeiro, Daniel Martins-de-Souza, Stevens K. Rehen
Scientific Reports October 9, 2017 DOI: 10.1038/s41598-017-12779-5 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Experimental study Peer reviewed |
|---|---|
| Population | Human cerebral organoids |
| Intervention | 5-MeO-DMT |
| Topics | Neuroplasticity 5-MeO-DMT DMT |
| Keywords | Psychedelic compounds psychedelics Serotonin-like molecule Psychoactive compounds Neuroscience brain health Brain activity Brain cell structure Neurobiology Brain development Cognition Memory Molecular mechanisms proteomics Protein analysis Cellular structure Anti-inflammatory effects Molecular insights Cell biology |
| Citations | 137 |
| Key findings | 5-MeO-DMT treatment differentially expressed 934 proteins in human cerebral organoids, with in silico analysis revealing modulatory effects on proteins associated with long-term potentiation, dendritic spine formation, and cytoskeletal reorganization. |
Abstract
Dimethyltryptamines are entheogenic serotonin-like molecules present in traditional Amerindian medicine recently associated with cognitive gains, antidepressant effects, and changes in brain areas related to attention. Legal restrictions and the lack of adequate experimental models have limited the understanding of how such substances impact human brain metabolism. Here we used shotgun mass spectrometry to explore proteomic differences induced by 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) on human cerebral organoids. Out of the 6,728 identified proteins, 934 were found differentially expressed in 5-MeO-DMT-treated cerebral organoids. In silico analysis reinforced previously reported anti-inflammatory actions of 5-MeO-DMT and revealed modulatory effects on proteins associated with long-term potentiation, the formation of dendritic spines, including those involved in cellular protrusion formation, microtubule dynamics, and cytoskeletal reorganization. Our data offer the first insight about molecular alterations caused by 5-MeO-DMT in human cerebral organoids.