Serotonin-Sensitive Adenylate Cyclase in Neural Tissue and Its Similarity to the Serotonin Receptor: A Possible Site of Action of Lysergic Acid Diethylamide
James A. Nathanson, Paul Greengard
Proceedings of the National Academy of Sciences March 1, 1974 DOI: 10.1073/pnas.71.3.797 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Laboratory study Peer reviewed |
|---|---|
| Population | Thoracic ganglia homogenates from an insect nervous system |
| Interventions | Serotonin D-lysergic acid diethylamide (LSD) 2-bromo-LSD cyproheptadine |
| Topics | LSD Serotonin |
| Keywords | Cyclase Adenylate kinase 5-HT Receptor Cyproheptadine Neurotransmission Quipazine Serotonin antagonists Endocrinology Biochemistry Pharmacology |
| Citations | 95 |
| Key points | A serotonin-sensitive adenylate cyclase in insect nervous tissue is competitively inhibited by LSD with an inhibitory constant of 5 nM, suggesting a direct link between serotonin receptors and this enzyme in serotonergic neurotransmission. |
Abstract
An adenylate cyclase (EC 4.6.1.1) that is activated specifically by low concentrations of serotonin has been identified in homogenates of the thoracic ganglia of an insect nervous system. The activation of this enzyme by serotonin was selectively inhibited by extremely low concentrations of D-lysergic acid diethylamide (LSD), 2-bromo-LSD, and cyproheptadine, agents which are known to block certain serotonin receptors in vivo . The inhibition was competitive with respect to serotonin, and the calculated inhibitory constant of LSD for this serotonin-sensitive adenylate cyclase was 5 nM. The data are consistent with a model in which the serotonin receptor of neural tissue is intimately associated with a serotonin-sensitive adenylate cyclase which mediates serotonergic neurotransmission. The results are also compatible with the possibility that some of the physiological effects of LSD may be mediated through interaction with serotonin-sensitive adenylate cyclase.
Comparable studies
Other experimental studies on LSD, most cited first.
| Study | Year | Design | Participants |
|---|---|---|---|
| Neural correlates of the LSD experience revealed by multimodal neuroimaging. Human participants | 2016 | Experimental study | |
| Crystal structure of an LSD-bound human serotonin receptor | 2017 | Experimental study with structural biology and molecular dynamics simulations | |
| Lysergic Acid Diethylamide: Sensitive Neuronal Units in the Midbrain Raphe Midbrain neurons containing serotonin | 1968 | Experimental study | |
| Connectome-harmonic decomposition of human brain activity reveals dynamical repertoire re-organization under LSD. Human brain | 2017 | Experimental study | |
| LSD-Assisted Psychotherapy in Patients with Terminal Cancer Cancer patients | 1973 | Experimental study | n = 60 |
Citations in the library
Cited by 2
Cites 3
- THE DISTRIBUTION AND METABOLISM OF LYSERGIC ACID DIETHYLAMIDE Annals of the New York Academy of Sciences March 1, 1957
- Some Serotoninlike Activities of Lysergic Acid Diethylamide Science July 20, 1956
- Effects of Hallucinogenic and Tranquilizing Drugs on Serotonin Evoked Uterine Contractions. Experimental Biology and Medicine 1956