Metabolites of N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine were separated and characterized, and metabolic routes in rat liver, kidney, and brain extracts included oxidative deamination, N-demethylation, O-demethylation, and N-oxidation.
Abstract
The use of a series of liquid chromatographic techniques involving cation-exchange, reverse-phase and normal-phase chromatography has permitted the separation and characterisation of a number of metabolites of the psychotomimetic indolealkylamines N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine which were isolated following incubation of these compounds with rat tissue extracts. In liver, kidney and brain tissue extracts the routes of metabolism identified included oxidative deamination, N-demethylation, O-demethylation and N-oxidation. The quantitative significance of individual routes of metabolism in these tissues was assessed using N,N-dimethyltryptamine as a substrate.