Study of metabolism of psychotomimetic indolealkylamines by rat tissue extracts using liquid chromatography.
B R Sitaram, R Talomsin, G L Blackman, W R Mcleod
Biochemical Pharmacology May 1, 1987 DOI: 10.1016/0006-2952(87)90117-1 (opens in new tab) via PubMed
Summary
AI-generated from the abstractUsing liquid chromatography techniques, metabolites of the psychotomimetic compounds N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine were separated and characterized after incubation with rat tissue extracts. In liver, kidney, and brain extracts, metabolic routes included oxidative deamination, N-demethylation, O-demethylation, and N-oxidation. The quantitative importance of each route was assessed using N,N-dimethyltryptamine as a substrate.
Study at a glance
| Characteristics | Laboratory study Peer reviewed |
|---|---|
| Population | Rat tissue extracts |
| Interventions | N N-dimethyltryptamine 5-methoxy-N |
| Citations | 23 |
| Key finding | Metabolites of N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine were separated and characterized, and metabolic routes in rat liver, kidney, and brain extracts included oxidative deamination, N-demethylation, O-demethylation, and N-oxidation. |
Abstract
The use of a series of liquid chromatographic techniques involving cation-exchange, reverse-phase and normal-phase chromatography has permitted the separation and characterisation of a number of metabolites of the psychotomimetic indolealkylamines N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine which were isolated following incubation of these compounds with rat tissue extracts. In liver, kidney and brain tissue extracts the routes of metabolism identified included oxidative deamination, N-demethylation, O-demethylation and N-oxidation. The quantitative significance of individual routes of metabolism in these tissues was assessed using N,N-dimethyltryptamine as a substrate.