Distribution of the hallucinogens N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine in rat brain following intraperitoneal injection: application of a new solid-phase extraction LC-APcI-MS-MS-isotope dilution method.
S A Barker, M A Littlefield-Chabaud, C David
Journal of Chromatography. B, Biomedical Sciences and Applications February 10, 2001 DOI: 10.1016/s0378-4347(00)00442-4 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Method development and demonstration Peer reviewed |
|---|---|
| Population | Rat brain tissue |
| Interventions | DMT 5-methoxy DMT |
| Dose | 5 mg/kg |
| Topics | 5-MeO-DMT DMT |
| Keywords | Hallucinogens:DMT:Psychoactive Substances:Psychedelics:Potent Substances:Compounds Neurochemistry:brain chemistry:neuropharmacology:neuroscience |
| Citations | 23 |
| Key points | The described SPE and LC-APcI-MS-MS-ID method is applicable for detecting and quantifying DMT and 5-methoxy DMT in discrete rat brain regions. |
Abstract
A method for the solid-phase extraction (SPE) and liquid chromatographic-atmospheric pressure chemical ionization-mass spectrometric-mass spectrometric-isotope dilution (LC-APcI-MS-MS-ID) analysis of the indole hallucinogens N,N-dimethyltryptamine (DMT) and 5-methoxy DMT (or O-methyl bufotenin, OMB) from rat brain tissue is reported. Rats were administered DMT or OMB by the intraperitoneal route at a dose of 5 mg/kg and sacrificed 15 min post treatment. Brains were dissected into discrete areas and analyzed by the methods described as a demonstration of the procedure's applicability. The synthesis and use of two new deuterated internal standards for these purposes are also reported.
Citations in the library
Cited by 14
- Psychedelic 5-methoxy-N,N-dimethyltryptamine: metabolism, pharmacokinetics, drug interactions, and pharmacological actions. Current Drug Metabolism October 1, 2010
- A narrative synthesis of research with 5-MeO-DMT. Journal of Psychopharmacology March 1, 2022
- Pharmacokinetics of N,N-dimethyltryptamine in Humans. European Journal of Drug Metabolism and Pharmacokinetics May 1, 2023
- Potentially hallucinogenic 5-hydroxytryptamine receptor ligands bufotenine and dimethyltryptamine in blood and tissues. Scandinavian Journal of Clinical and Laboratory Investigation 2005
- 5-Methoxy-N,N-diisopropyltryptamine (Foxy), a selective and high affinity inhibitor of serotonin transporter. Toxicology Letters April 5, 2007
and 9 more in the library
Cites 14
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- The psychopharmacology of hallucinogens. Neuropsychopharmacology April 1, 1996
- Pharmahuasca: Human Pharmacology of Oral DMT Plus Harmine Journal of Psychoactive Drugs April 1, 1999
- In vivo metabolism of 5-methoxy-N,N-dimethyltryptamine and N,N-dimethyltryptamine in the rat. Biochemical Pharmacology May 1, 1987
- Hallucinogenic N-methylated indolealkylamines in the cerebrospinal fluid of psychiatric and control populations. The British Journal of Psychiatry February 1, 1978
and 9 more in the library