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bioRxiv (Cold Spring Harbor Laboratory)

209 papers in the library · 590 citations · publishing 2015-2026

Papers

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Intravenous Administration of Serotonergic Psychedelics Produce Short-lasting Changes in Sleep-Wake Behavior and High Gamma Functional Connectivity in Rats

bioRxiv (Cold Spring Harbor Laboratory) October 14, 2025 Nicholas Kolbman, Amanda Nelson, Rachel Summerfield et al. 1 citation preprint

Abstract Background and Purpose Given the increase in recreational psychedelic use and ongoing efforts to explore psychedelics as therapeutic agents for mental health disorders, there is an urgent need to understand the effect of psychedelics such as psilocybin and N,N-dimethyltryptamine (DMT) on sleep-wake states, which share a bidirectional relationship with mental health. Here, we...

Disrupted hierarchical organization in disorders of consciousness revealed by fluctuation-dissipation deviations

bioRxiv (Cold Spring Harbor Laboratory) October 3, 2025 Marian Martínez-Marín, Jakub Vohryzek, Anira Escrichs et al. 1 citation preprint

Abstract Evaluating consciousness levels after coma remains clinically challenging, and probing the brain’s functional hierarchy offers model-based biomarkers of brain states. We characterize the hierarchy loss in disorders of consciousness (DoC) via departures from non-equilibrium dynamics. Irreversible, directed interactions are indexed by deviation from the fluctuation– dissipation theorem...

Vividness of mental imagery reflects a broad range of internally generated visual experiences

bioRxiv (Cold Spring Harbor Laboratory) October 2, 2025 D. Samuel Schwarzkopf, Xinran A Yu, Ecem Altan et al. preprint

Abstract Research on mental visual imagery typically relies on vividness ratings. However, vividness is ill-defined as it lacks an objective reference. Here, we present survey results that suggest vividness is nevertheless a robust measure. It explains individual differences of a broad range of subjective experiences, from the detail of mental imagery, the propensity to report having other...

Ketamine-induced NMDA receptor hypofunction alters social and locomotor behavior in adult zebrafish

bioRxiv (Cold Spring Harbor Laboratory) September 17, 2025 Matheus Gallas‐lopes, Dagobert Müller, Thailana Stahlhofer-Buss et al. preprint

ABSTRACT Background NMDA receptor antagonists, such as ketamine, are widely used to model schizophrenia-related phenotypes in preclinical studies. While zebrafish have emerged as a promising model organism for neuropsychiatric research, few studies have characterized their behavioral responses to repeated ketamine exposure. Methods Three independent experiments were conducted to evaluate the...

Effective connectivity of the human claustrum: Triple networks, subcortical circuits, and psychedelic modulation

bioRxiv (Cold Spring Harbor Laboratory) September 12, 2025 Navid Shams Masjedi, Adeel Razi preprint

Abstract Decades of cross-species research highlight the claustrums extensive bidirectional connectivity with cortical and subcortical regions, implicating it in higher-order cognitive processes requiring synchronized brain states. Psychedelics may disrupt this synchrony by modulating claustro-cortical signaling, reflected by the dissolution of cortical network signatures. Using spectral...

EEG Phase Slips Reveal Detailed Brain Activity Patterns of Novice Vipassana Meditators During Decision Making Tasks

bioRxiv (Cold Spring Harbor Laboratory) September 9, 2025 Ratna Jyothi Kakumanu, Ajay Kumar Nair, Arun Sasidharan et al. preprint

Abstract Our study adopted a novel approach, utilizing four biomarkers, namely EEG potentials, their first-order derivatives, and phase slip rates derived from each, to discern the differences between novice Vipassana (NVP) subjects and non-meditator controls (NMC). Phase slip rates are discontinuities in instantaneous phase that represent cortical phase transitions, indicating a significant...

Immersive NREM dreaming preserves subjective sleep depth against declining sleep pressure

bioRxiv (Cold Spring Harbor Laboratory) September 8, 2025 Adriana Michalak, Davide Marzoli, Francesco Pietrogiacomi et al. preprint

Perceived sleep depth is a key determinant of subjective sleep quality, traditionally thought to reflect unconsciousness and reduced cortical activation. Here, we combined high-density EEG with a serial awakening paradigm during NREM sleep to examine its neural and experiential correlates. As expected, deeper sleep was associated with reduced cortical activation, reflected in a lower...

Live access to the emotional dynamics of REM sleep dreams in lucid dreamers with narcolepsy

bioRxiv (Cold Spring Harbor Laboratory) September 1, 2025 Jean-Baptiste Maranci, Pierre Champetier, Delphine Oudiette et al. preprint

Abstract Sleep helps regulate emotions, but it is still unclear whether -and how- the emotions we experience in dreams contribute to this regulation. To uncover the potential function of dream emotions, we must first understand what they are and how they unfold in dreams. The emotional content of dreams has mostly been studied using post-sleep dream reports, which provide a biased and static...

Context-dependent structurally informed effective connectivity under psilocybin

bioRxiv (Cold Spring Harbor Laboratory) August 22, 2025 Matthew D. Greaves, Tamrin Barta, Leonardo Novelli et al. preprint

Abstract The extent to which anatomical connectivity constrains pharmacologically altered brain dynamics remains poorly understood. Here, we combined psilocybin administration with a structurally informed effective-connectivity model to examine how structural connectivity shapes directed inter-regional influences across experiential contexts. Using dynamic causal modeling embedded in a...

Psilocybin Prolongs the Neurovascular Coupling Response in Mouse Visual Cortex

bioRxiv (Cold Spring Harbor Laboratory) July 31, 2025 Rick Zirkel, Matthew Isaacson, Clara Liao et al. 3 citations preprint

Abstract Psilocybin has profound therapeutic potential for various mental health disorders, but its mechanisms of action are unknown. Functional MRI studies have reported the effects of psilocybin on brain activity and connectivity; however, these measurements rely on neurovascular coupling to infer neural activity changes and assume that blood flow responses to neural activity are not altered...

Modular dynamics of conscious and unconscious states in marmoset cortex

bioRxiv (Cold Spring Harbor Laboratory) July 15, 2025 Dominic Standage, Yuki Hori, Joseph Y. Nashed et al. preprint

General anesthetics are routinely used to induce unconsciousness. While much is known about their effects on receptor function and the activity of individual neurons, much less is known about how these local effects are manifest at the level of largescale, distributed brain networks. Using functional magnetic resonance imaging (fMRI) with the common marmoset (Callithrix jacchus) we investigated...

Psilocybin has no immediate or persistent analgesic effect in acute and chronic mouse pain models

bioRxiv (Cold Spring Harbor Laboratory) July 7, 2025 Akila Ram, Austen B Casey, Robert C. Malenka et al. 2 citations preprint

Abstract The psychedelic psilocybin may have lasting therapeutic effects for patients with chronic pain syndromes. Some clinical and preclinical data suggest these putative benefits derive from direct analgesic effects. However, this possibility has not been comprehensively tested in preclinical models. Here, we show that psilocybin is not analgesic over a range of doses across multiple pain...

Distinct patterns of directed brain connectivity in focused attention, open monitoring and loving kindness meditation: An EEG Granger causality study with long-term meditators

bioRxiv (Cold Spring Harbor Laboratory) July 4, 2025 Vasil Kolev, Kosio Beshkov, Peter Malinowski et al. 2 citations preprint

ABSTRACT The present study applied spectral Granger causality analysis to electroencephalographic (EEG) recordings obtained during Focused Attention Meditation (FAM), Open Monitoring Meditation (OMM), and Loving Kindness Meditation (LKM) in highly experienced meditators. The aim of the investigation was to uncover distinct connectivity signatures associated with each meditation style by...

Nightly dynamics of emotional content in dreams

bioRxiv (Cold Spring Harbor Laboratory) June 28, 2025 Jessica Palmieri, Valentina Elce, Monika Schönauer 1 citation preprint

Abstract Emotional processing is a crucial adaptive function. Research suggests that sleep, particularly rapid eye movement (REM) sleep, may have a role in processing the emotional load of past events. Notably, dream experiences may offer insight into this nighttime process. Some studies have reported increased emotionality in dreams as the night progresses, possibly reflecting ongoing...

Similar States, Different Paths: Neurodynamics of diverse meditation techniques

bioRxiv (Cold Spring Harbor Laboratory) June 26, 2025 Prakash Shrimali, Arun Sasidharan, Saketh Malipeddi et al. 1 citation preprint

Abstract Meditation encompasses diverse practices that train attention inward, in contrast to externally oriented task states. However, the neurodynamic features distinguishing meditative states from non-meditative states across traditions remain unclear. We analyzed high-density EEG data (N=170; 121 advanced meditators, 49 controls) across four traditions: Vipassana, Brahma Kumaris Raja Yoga,...

Selective transcriptomic recovery by ( 2R,6R )-hydroxynorketamine in opioid-abstinent mice: Machine learning identifies predictive biomarkers

bioRxiv (Cold Spring Harbor Laboratory) June 8, 2025 Anna Onisiforou, Morfeas Koumas, Andria Michael et al. preprint

Abstract Background Opioid abstinence induces persistent emotional disturbances and widespread neuroplastic changes, in areas of the brain including the hippocampus. Although ketamine and its metabolite ( 2R,6R )-hydroxynorketamine (HNK) show potential in reversing opioid abstinence-related deficits in rodents, the molecular mechanisms underlying their efficacy remain poorly understood. Methods...

Receptor-enriched analysis of functional connectivity (REACT) for understanding cannabinoid neuropsychopharmacology

bioRxiv (Cold Spring Harbor Laboratory) June 5, 2025 Ekaterina Shatalina, Natalie Ertl, Kat Petrilli et al. 1 citation preprint

Abstract Background Cannabis is one of the most widely used psychoactive substances in the world and is increasingly investigated as a treatment for neuropsychiatric conditions. Cannabis contains delta-9-tetrahydrocannabinol (THC), which is thought to underlie its main psychoactive effects, and cannabidiol (CBD), which has been proposed to modulate the effects of THC on the brain. Both have...

Attrition Rates in Mindfulness-Based Interventions for Chronic Pain: A Meta-Analysis

bioRxiv (Cold Spring Harbor Laboratory) May 30, 2025 Michael Y. Wang, M. Prabhavi N. Perera, Paul B. Fitzgerald et al. 2 citations preprint

Abstract Objective Mindfulness-based interventions (MBIs) show promise in managing chronic pain but often require substantial time commitments, leading to high attrition rates and concerns about acceptability. This meta-analysis evaluated attrition rates in MBIs for chronic pain and explored factors contributing to participant withdrawal. Methods Following PRISMA guidelines, 44 studies (45...

Converging pathways: shared brain circuitry engaged by monoaminergic antidepressants, ketamine and psilocybin

bioRxiv (Cold Spring Harbor Laboratory) May 30, 2025 Pavel Osten, K Sunny Joseph, Jane Collins et al. preprint

Abstract Ketamine has transformed depression treatment by providing therapeutic relief within a single day, unlike monoaminergic antidepressants that require weeks to take effect. Here, we conducted whole-brain screening in mice to compare drug-evoked c-fos expression—acting as a marker of brain activity leading to protein synthesis-dependent forms of plasticity—following treatment with...

The semantics of dreams

bioRxiv (Cold Spring Harbor Laboratory) May 21, 2025 Valentina Elce, Giorgia Bontempi, Serena Scarpelli et al. 1 citation preprint

Dreams are universal yet deeply personal experiences. While memory and personal concerns influence dream content, the impact of other individual, generalizable traits remains poorly understood. To address this gap, we built a multimodal dataset including dream and wakefulness reports, alongside demographic, psychometric, cognitive, and sleep-related measures in a large adult cohort. Natural...

Longer chronic cannabis use in humans is associated with impaired implicit motor learning and supranormal resting state cortical activity

bioRxiv (Cold Spring Harbor Laboratory) April 21, 2025 Shikha Prashad, Andrew Paek, Lisa R. Fournier 1 citation preprint

Abstract Chronic cannabis use is associated with cognitive impairment, but its impact on implicit motor learning is unclear. Implicit learning of movement sequences (i.e., their specific ordinal and temporal structure) is vital for performing complex motor behavior and lays the foundation for performing daily activities and interacting socially. We collected data from 30 individuals who used...

PsiConnect: A Multimodal Neuroimaging Study of Psilocybin-Induced Changes in Brain and Behaviour

bioRxiv (Cold Spring Harbor Laboratory) April 14, 2025 Leonardo Novelli, Devon Stoliker, Tamrin Barta et al. 3 citations preprint

ABSTRACT PsiConnect is a large-scale neuroimaging study designed to investigate the neural and subjective effects of psilocybin using multimodal neuroimaging. It combines functional, structural, and diffusion-weighted MRI with EEG to examine brain activity in 62 participants before and after a 19 mg dose of psilocybin. The design includes resting-state scans and three naturalistic conditions:...

A multi-institutional investigation of psilocybin’s effects on mouse behavior

bioRxiv (Cold Spring Harbor Laboratory) April 9, 2025 Odilia D Lu, Katrina White, Kendall Raymond et al. 18 citations preprint

ABSTRACT Studies reporting novel therapeutic effects of psychedelic drugs are rapidly emerging. However, the reproducibility and reliability of these findings could remain uncertain for years. Here, we implemented a multi-institutional collaborative approach to define the robust and replicable effects of the psychedelic drug psilocybin on mouse behavior. Five laboratories performed the same...

Cross-Frequency Coupling as a Neural Substrate for Prediction Error Evaluation: A Laminar Neural Mass Modeling Approach

bioRxiv (Cold Spring Harbor Laboratory) March 19, 2025 Giulio Ruffini, Edmundo Lopez-Sola, Raul P. Aristides et al. 8 citations preprint

Abstract Predictive coding frameworks suggest that neural computations rely on hierarchical error minimization, where sensory signals are evaluated against internal model predictions. However, the neural implementation of this inference process remains unclear. We propose that cross-frequency coupling (CFC) furnishes a fundamental mechanism for this form of inference. We first demonstrate that...

Reactivating a relaxation exercise during sleep to influence cortical hyperarousal in people with frequent nightmares – a randomized crossover trial

bioRxiv (Cold Spring Harbor Laboratory) March 13, 2025 Clara Sayk, Alicia Probst, F. F. Lange et al. preprint

Study Objectives High-frequency EEG activity during sleep (cortical hyperarousal), is a transdiagnostic feature across psychiatric disorders, including nightmare disorder. It is discussed as a target of intervention; however, specific treatment options are yet unavailable. We tested whether exposure to relaxation-associated odor cues during sleep would reduce cortical hyperarousal, i.e. beta...