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Stevens K. Rehen

12 papers in the library · 281 citations · publishing 2016-2026

Papers

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Proliferative Effects of the Psychedelic N,N-Dimethyltryptamine (DMT) in Human Neural Stem Cells.

ACS Chemical Neuroscience July 9, 2026 José Alexandre Salerno, Elizabeth R. Dominguez, Karina Karmirian et al.

The serotonergic psychedelic N,N-dimethyltryptamine (DMT) produces rapid antidepressant effects in preclinical and early clinical studies. Therapeutic benefits have been linked to sustained neural plasticity, including adult neurogenesis in rodents. Whether brief DMT exposure engages proliferative responses in human neural stem cells (NSCs) remains unresolved. Using human iPSC-derived NSCs, we...

Lysergic Acid Diethylamide extends lifespan in Caenorhabditis elegans

bioRxiv Preprint Server June 16, 2025 Beatriz de S. Carrilho, Aline Duarte, Isabelle Martins et al. preprint

Aging is modulated by nutrient-sensing pathways that integrate metabolic and hormonal cues to regulate growth, stress resilience, and lifespan. Caloric restriction (CR), a well-established intervention, extends longevity in diverse species through modulation of conserved nutrient-sensing pathways, including TOR signaling. Lysergic acid diethylamide (LSD), a classic serotonergic psychedelic with...

LSD Modulates Proteins Involved in Cell Proteostasis, Energy Metabolism and Neuroplasticity in Human Cerebral Organoids.

ACS Omega August 27, 2024 Marcelo N. Costa, Livia Goto-Silva, Juliana Nascimento et al. 3 citations

Proteomic analysis of human cerebral organoids may reveal how psychedelics regulate biological processes, shedding light on drug-induced changes in the brain. This study elucidates the proteomic alterations induced by lysergic acid diethylamide (LSD) in human cerebral organoids. By employing high-resolution mass spectrometry-based proteomics, we quantitatively analyzed the differential...

Lysergic acid diethylamide induces behavioral changes in Caenorhabditis elegans.

Neuroscience Letters August 10, 2024 Isis M. Ornelas, Beatriz de S. Carrilho, Matheus Antonio V. de C. Ventura et al. 3 citations

Lysergic acid diethylamide (LSD) is a synthetic psychedelic compound with potential therapeutic value for psychiatric disorders. This study aims to establish Caenorhabditis elegans as an in vivo model for examining LSD's effects on locomotor behavior. Our results demonstrate that LSD is absorbed by C. elegans and that the acute treatment reduces animal speed, similar to the role of endogenous...

Human pluripotent stem cells as a translational toolkit in psychedelic research in vitro.

iScience March 28, 2024 José Alexandre Salerno, Stevens K. Rehen 4 citations

Psychedelics, recognized for their impact on perception, are resurging as promising treatments with rapid onset for mood and substance use disorders. Despite increasing evidence from clinical trials, questions persist about the cellular and molecular mechanisms and their precise correlation with treatment outcomes. Murine neurons and immortalized non-neural cell lines harboring overexpressed...

Nootropic effects of LSD: Behavioral, molecular and computational evidence.

Experimental Neurology June 1, 2022 Isis M. Ornelas, F. A. Cini, Isabel Wießner et al. 37 citations

The therapeutic use of classical psychedelic substances such as d-lysergic acid diethylamide (LSD) surged in recent years. Studies in rodents suggest that these effects are produced by increased neural plasticity, including stimulation of the mTOR pathway, a key regulator of metabolism, plasticity, and aging. Could psychedelic-induced neural plasticity be harnessed to enhance cognition? Here we...

Proteomic changes induced by harmine in human brain organoids reveal signaling pathways related to neuroprotection

bioRxiv (Cold Spring Harbor Laboratory) June 17, 2021 Karina Karmirian, Livia Goto-Silva, Juliana Nascimento et al. 1 citation preprint

Abstract Harmine is a β-carboline found in Banisteriopsis caapi , a constituent of ayahuasca brew. Ayahuasca is consumed as a beverage in native Americans’ sacred rituals and in religious ceremonies in Brazil. Throughout the years, the beneficial effects of ayahuasca to improve mental health and life quality have been reported, which propelled the investigation of its therapeutic potential to...

d-Lysergic acid diethylamide has major potential as a cognitive enhancer

bioRxiv (Cold Spring Harbor Laboratory) December 6, 2019 Felipe Augusto Cini da Silva, Isis M. Ornelas, Encarni Marcos et al. 9 citations preprint

Abstract Psychedelic agonists of serotonin receptors induce neural plasticity and synaptogenesis, but their potential to enhance learning remains uncharted. Here we show that a single dose of d-LSD, a potent serotonergic agonist, increased novel object preference in young and adult rats several days after treatment. d-LSD alone did not increase preference in old animals, but could rescue it to...

Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT.

Scientific Reports October 9, 2017 Vanja Dakic, Juliana Minardi Nascimento, Rafaela Costa Sartore et al. 137 citations

Dimethyltryptamines are entheogenic serotonin-like molecules present in traditional Amerindian medicine recently associated with cognitive gains, antidepressant effects, and changes in brain areas related to attention. Legal restrictions and the lack of adequate experimental models have limited the understanding of how such substances impact human brain metabolism. Here we used shotgun mass...

Short term changes in the proteome of human cerebral organoids induced by 5-methoxy-N,N-dimethyltryptamine

bioRxiv (Cold Spring Harbor Laboratory) February 13, 2017 Vanja Dakic, Juliana Nascimento, Rafaela Sartore et al. preprint

Abstract Dimethyltryptamines are hallucinogenic serotonin-like molecules present in traditional Amerindian medicine (e.g. Ayahuasca) recently associated with cognitive gains, antidepressant effects and changes in brain areas related to attention. Historical and technical restrictions impaired understanding how such substances impact human brain metabolism. Here we used shotgun mass spectrometry...

Harmine stimulates proliferation of human neural progenitors

PeerJ December 6, 2016 Vanja Dakic, Renata de Moraes Maciel, Hannah Drummond et al. 86 citations

Harmine is the β -carboline alkaloid with the highest concentration in the psychotropic plant decoction Ayahuasca. In rodents, classical antidepressants reverse the symptoms of depression by stimulating neuronal proliferation. It has been shown that Ayahuasca presents antidepressant effects in patients with depressive disorder. In the present study, we investigated the effects of harmine in...

Harmine stimulates neurogenesis of human neural cells in vitro

April 14, 2016 Vanja Dakic, Renata de Moraes Maciel, Hannah Drummond et al. 1 citation

Harmine is a β-carboline alkaloid present at highest concentration in the psychotropic plant decoction Ayahuasca. In rodents, classical antidepressants reverse the symptoms of depression by stimulating neuronal proliferation. It has been shown that Ayahuasca presents antidepressant effects in patients with depressive disorder. In the present study, we investigated the effects of harmine in cell...