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Tim Schäfer

7 papers in the library · 93 citations · publishing 2022-2025

Papers

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Dissimilar Reactions and Enzymes for Psilocybin Biosynthesis in Inocybe and Psilocybe Mushrooms

Angewandte Chemie International Edition September 21, 2025 Tim Schäfer, Fabian Haun, Bernhard Rupp et al. 7 citations

Abstract Psilocybin (4‐phosphoryloxy‐ N , N ‐dimethyltryptamine, 1 ) is the main indolethyl‐amine natural product of psychotropic (so‐called “magic”) mushrooms. The majority of 1 ‐producing species belongs to the eponymous genus Psilocybe , for which the biosynthetic events, beginning from l ‐tryptophan ( 2 ), and the involved enzymes have thoroughly been characterized. Some Inocybe (fiber cap)...

Unterschiedliche Reaktionen und Enzyme in der Psilocybin‐ Biosynthese bei Inocybe‐ und Psilocybe ‐Pilzen

Angewandte Chemie September 21, 2025 Tim Schäfer, Fabian Haun, Bernhard Rupp et al.

Zusammenfassung Psilocybin (4‐Phosphoryloxy‐ N , N ‐dimethyltryptamin, 1 ) ist der hauptsächliche Indolethylamin‐Naturstoff der psychotropen sogenannten Zauberpilze. Die Mehrheit der 1 ‐produzierenden Arten gehört zu der namensgebenden Gattung Psilocybe , für die die biosynthetischen Ereignisse, ausgehend von l ‐Tryptophan ( 2 ), sowie die beteiligten Enzyme gründlich charakterisiert wurden....

In Vitro Psilocybin Synthesis by Co‐Immobilized Enzymes

Chemistry - A European Journal April 9, 2025 Tim Schäfer, Thomas Krüger, Jakob Worbs et al. 2 citations

Abstract Advanced clinical trials investigate the Psilocybe magic mushroom natural product psilocybin as a treatment against major depressive disorder. Currently, synthetic material is used to meet the demand for legitimate pharmaceutical purposes. Here, we report an in vitro approach to biocatalytically produce psilocybin on a solid‐phase matrix charged with five covalently bound biosynthetic...

Optimized psilocybin production in tryptophan catabolism‐repressed fungi

Microbial Biotechnology November 1, 2024 Slavica Janevska, Sophie Weiser, Ying Huang et al. 13 citations

Abstract The high therapeutic potential of psilocybin, a prodrug of the psychotropic psilocin, holds great promise for the treatment of mental disorders such as therapy‐refractory depression, alcohol use disorder and anorexia nervosa. Psilocybin has been designated a ‘Breakthrough Therapy’ by the US Food and Drug Administration, and therefore a sustainable production process must be established...

Genetic regulation of L-tryptophan metabolism in Psilocybe mexicana supports psilocybin biosynthesis.

Fungal biology and biotechnology April 25, 2024 Paula Sophie Seibold, Sebastian Dörner, Janis Fricke et al. 8 citations

Although Basidiomycota produce pharmaceutically and ecologically relevant natural products, knowledge of how they coordinate their primary and secondary metabolism is virtually non-existent. Upon transition from vegetative mycelium to carpophore formation, mushrooms of the genus Psilocybe use L-tryptophan to supply the biosynthesis of the psychedelic tryptamine alkaloid psilocybin with the...

Characterization of the Gateway Decarboxylase for Psilocybin Biosynthesis

ChemBioChem November 3, 2022 Tim Schäfer, Kristina Kramer, Sebastiaan Werten et al. 20 citations

Abstract The l ‐tryptophan decarboxylase PsiD catalyzes the initial step of the metabolic cascade to psilocybin, the major indoleethylamine natural product of the “magic” mushrooms and a candidate drug against major depressive disorder. Unlike numerous pyridoxal phosphate (PLP)‐dependent decarboxylases for natural product biosyntheses, PsiD is PLP‐independent and resembles type II...

Genetic Survey of Psilocybe Natural Products

ChemBioChem May 18, 2022 Sebastian Dörner, Kai Rogge, Janis Fricke et al. 43 citations

Abstract Psilocybe magic mushrooms are best known for their main natural product, psilocybin, and its dephosphorylated congener, the psychedelic metabolite psilocin. Beyond tryptamines, the secondary metabolome of these fungi is poorly understood. The genomes of five species ( P. azurescens , P. cubensis , P. cyanescens , P. mexicana , and P. serbica ) were browsed to understand more profoundly...