Ru-Yi Cai

Selective GRAB sensors reveal distinct endocannabinoid dynamics in vivo

bioRxiv August 12, 2026 Ru-Yi Cai, Yue-Qi Yang, Shang-Xuan Cai et al. preprint

The endocannabinoid system modulates diverse physiological processes via two endogenous lipid ligands, 2-arachidonoylglycerol (2-AG) and anandamide (AEA); however, their specific spatiotemporal dynamics remain poorly understood owing to the lack of selective probes. Here, we developed GRAB2-AG2.0 and GRABAEA2.0, two genetically encoded fluorescent sensors that selectively detect 2-AG and AEA,...