Determination of ibotenic acid and muscimol in Amanita muscaria by high performance liquid chromatography.
S. Komiyama, Y. Yamaura, H. Nakazawa, M. Fujita, Yozo Kabasawa
Study at a glance
AI-extracted from the abstract| Characteristics | Analytical method development study |
|---|---|
| Key points | Reports an HPLC method that separates and simultaneously quantifies ibotenic acid and muscimol using an aminopropyl-bonded column, phosphate buffer–methanol mobile phase, and 220 nm detection. The authors state that pretreatment with a layered silica gel column (anion-exchange resin plus alumina) removes interfering substances, enabling accurate simultaneous determination of both compounds. |
Abstract
毒キノコのベニテングタケに含有され,中枢神経系に作用するイソキサゾール化合物のイボテン酸とムッシモールの高速液体クロマトグラフィーによる分析法を検討した。カラムはアミノプロピル基を化学結合したShim-pack P NH2-10/S2504を用い,0.01Mリン酸塩緩衝液(pH4.5)-メタノール(30:70)を移動相とし,220nmで検出した。陰イオン交換樹脂及びアルミナを積層したシリカゲルカラムによる前処理を行うことにより混在物の影響もなく,精度良くイボテン酸,ムッシモールの同時定量が可能である。
Citations in the library
Cited by 3
- Amanita muscaria: chemistry, biology, toxicology, and ethnomycology. Mycological research February 1, 2003
- Theoretical and experimental NMR studies on muscimol from fly agaric mushroom (Amanita muscaria). Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy January 15, 2016
- Hallucinogenic species in Amanita muscaria. Determination of muscimol and ibotenic acid by ion-interaction HPLC. Journal of Liquid Chromatography & Related Technologies 1997