Development of a RP-HPLC Method for Separating and Quantifying Muscimol in Different Developmental Stages of the Fungus Amanita muscaria
Emerson León, James Guevara‐Pulido
Journal of Chemistry November 20, 2020 DOI: 10.1155/2020/8859998 (opens in new tab) via OpenAlex
Summary
AI-generated from the abstractA reverse-phase high-performance liquid chromatography method was developed to quantify muscimol in five aqueous extracts of Amanita muscaria at different developmental stages. The highest concentration of muscimol (1,210 mg/ml) occurred in the young mushroom stage, and concentrations progressively decreased as the fungus aged. The method offers a simple means to quantify muscimol, a metabolite of pharmaceutical interest as a possible treatment for tardive dyskinesia and Parkinson's disease.
Study at a glance
| Characteristics | Quantitative analytical method development Peer reviewed |
|---|---|
| Population | Amanita muscaria mushrooms at five developmental stages |
| Keywords | Muscimol Amanita Fungus Tardive dyskinesia Metabolite |
| Citations | 3 |
| Key finding | The maximum concentration of muscimol (1,210 mg/ml) was found in the young mushroom stage, with concentrations decreasing as the fungus ages. |
Abstract
A simple RP-HPLC method was designed for the quantification of muscimol (5-(aminomethyl)-isoxazol-3-ol) present in five aqueous extracts of Amanita muscaria each from a different developmental stage. Results show that the maximum concentration of muscimol (1,210 mg/ml) was found in the young mushroom stage of development. Moreover, it was also found that this concentration progressively decreases as the fungus ages. The developed method is a simple but effective method for the quantification of muscimol, a widely important metabolite for the pharmaceutical industry as a possible treatment for tardive dyskinesia and Parkinson’s disease.