Determination of LSD in Urine With High-Performance Liquid Chromatography–Mass Spectrometry
Therapeutic Drug Monitoring August 1, 2001 DOI: 10.1097/00007691-200108000-00011 (opens in new tab) via OpenAlex
Summary
AI-generated from the abstractA method using high-performance liquid chromatography and mass spectrometry can reliably detect LSD in urine at concentrations as low as 0.02 ng/mL. The procedure involves extracting LSD from urine into an organic solvent, then back-extracting into an acetate buffer, followed by separation and detection. A trideuterated form of LSD serves as an internal standard to improve accuracy. The method shows consistent results, with measurement variability of 3.5% within a day and 4.0% between days, and recovers 91% of LSD from samples. The calibration curve is linear across 0.05 to 10 ng/mL, making it suitable for routine testing.
Study at a glance
| Characteristics | Method development and validation Peer reviewed |
|---|---|
| Keywords | Mass spectrometry Detection limit Extraction chemistry High-performance liquid chromatography Electrospray ionization |
| Citations | 11 |
| Key finding | The developed method can detect LSD in urine down to 0.02 ng/mL with high precision and recovery. |
Abstract
A rapid, specific, and sensitive high-performance liquid chromatography/mass spectrometry method has been developed for routine determination of lysergic acid diethylamide (LSD) in urine. It includes sample purification by extraction into an organic solvent and back-extraction to an acetate buffer, reversed-phase high-performance liquid chromatography, and detection with a single quadrupole mass spectrometer equipped with an atmospheric pressure ionization electrospray interface. Trideuterated LSD was used as internal standard. The limit of detection was 0.02 ng/mL and the calibration curve was linear from 0.05 to 10 ng/mL. Within-and between-day coefficients of variation were 3.5% and 4.0% respectively and extraction recovery was 91%.