Detection of Lysergic Acid Diethylamide (LSD) in Urine by Gas Chromatography-Ion Trap Tandem Mass Spectrometry
J. H. Sklerov, Kathryn S. Kalasinsky, C. A. Ehorn
Journal of Analytical Toxicology October 1, 1999 DOI: 10.1093/jat/23.6.474 (opens in new tab) via OpenAlex
Summary
AI-generated from the abstractA confirmatory method using gas chromatography-tandem mass spectrometry (GC-MS-MS) with an internal ionization ion trap detector can detect and quantify lysergic acid diethylamide (LSD) in urine. After a single-step solid-phase extraction of 5 mL of urine, underivatized LSD is measured with limits of quantitation and detection of 80 and 20 pg/mL, respectively. Temperature-programmed on-column injections show linearity over 20-2000 pg/mL (r² = 0.999). Intraday and interday coefficients of variation are below 6% and 13%, respectively. The method has been applied to quality-control specimens and LSD-positive samples, with comparisons to alternative GC-MS methods and extraction procedures.
Study at a glance
| Characteristics | Method development and validation Peer reviewed |
|---|---|
| Population | Urine samples (quality-control and LSD-positive) |
| Topics | LSD |
| Keywords | Urine Detection limit Tandem mass spectrometry Gas chromatography–mass spectrometry Ion trap |
| Citations | 19 |
| Key finding | A GC-MS-MS method using an ion trap detector can detect LSD in urine at concentrations as low as 20 pg/mL with high precision and linearity. |
Abstract
A confirmatory method for the detection and quantitation of lysergic acid diethylamide (LSD) is presented. The method employs gas chromatography-tandem mass spectrometry (GC-MS-MS) using an internal ionization ion trap detector for sensitive MS-MS-in-time measurements of LSD extracted from urine. Following a single-step solid-phase extraction of 5 mL of urine, underivatized LSD can be measured with limits of quantitation and detection of 80 and 20 pg/mL, respectively. Temperature-programmed on-column injections of urine extracts were linear over the concentration range 20-2000 pg/mL (r2 = 0.999). Intraday and interday coefficients of variation were < 6% and < 13%, respectively. This procedure has been applied to quality-control specimens and LSD-positive samples in this laboratory. Comparisons with alternate GC-MS methods and extraction procedures are discussed.