Determination of Lysergic Acid Diethylamide (LSD) in Urine by Instrumental High-Performance Thin-Layer Chromatography
Lee M. Blum, E F Carenzo, Fredric Rieders
Journal of Analytical Toxicology September 1, 1990 DOI: 10.1093/jat/14.5.285 (opens in new tab) via OpenAlex
Summary
AI-generated from the abstractA high-performance thin-layer chromatography method can detect less than one microgram of LSD per liter of urine after a single-step extraction with an alkaline wash. The technique is compared with radioimmunoassay for measuring LSD in urine.
Study at a glance
| Characteristics | Method development and comparison Peer reviewed |
|---|---|
| Topics | LSD |
| Keywords | Urine Extraction chemistry Radioimmunoassay Thin-layer chromatography |
| Citations | 15 |
| Key finding | The HPTLC technique can detect less than 1 microgram of LSD per liter of urine. |
Abstract
An instrumental high-performance thin-layer chromatographic (HPTLC) technique for the determination of lysergic acid diethylamide (LSD) in urine was developed. Before chromatographic separation, a single-step extraction with alkaline wash is performed. The procedure can detect less than 1 microgram LSD/L urine. Results of HPTLC determination are compared with those from a radioimmunoassay (RIA) procedure.