[Authentication and ultra performance liquid chromatography (UPLC)/MS analysis of magic mint, Salvia divinorum and its related plants].
Takuro Maruyama, Hiroyuki Kamakura, Ruri Kikura-Hanajiri, Yukihiro Goda
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan 2008 DOI: 10.1248/yakushi.128.179 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Analytical study Peer reviewed |
|---|---|
| Population | Commercial Salvia cultivars available in the Japanese market and Salvia divinorum leaves and processed products |
| Topics | Salvia divinorum |
| Keywords | Magic mint Psychoactive plant Hallucinogenic plant Plant identification Genetic testing Species identification Botanical identification Plant authentication Plant analysis Plant classification Regulatory tool Drug regulation Plant regulation Substance control Legal control Regulatory science Drug enforcement Hallucinogenic compound Psychoactive compound Active ingredient Chemical analysis |
| Citations | 14 |
| Key points | None of the commercial Salvia cultivars contained salvinorin A, while S. divinorum leaves and its processed products contained 0.19% to 0.58% of the compound. |
Abstract
Ultra performance liquid chromatography (UPLC)/mass spectrometry (MS) analysis was performed to investigate whether commercial Salvia cultivars available in the Japanese market contain salvinorin A (1), which is an hallucinogen present in magic mint (Salvia divinorum) prior to the regulation of S. divinorum by the Japanese Pharmaceutical Affairs Law. In addition, a previously reported method to authenticate S. divinorum, utilizing an amplification refractory mutation system (ARMS) was applied to the same samples to estimate the method's accuracy. As a result of the UPLC/MS analysis, it was clear that none of the tested cultivars possessed 1 while S. divinorum leaves and its processed products "concentrated salvia" contained 1 in the range from 0.19% to 0.58%. Furthermore, the ARMS method could clearly distinguish S. divinorum from the tested cultivars. In conclusion, the authentication method is considered to be useful for the practical regulation of S. divinorum due to its simplicity and accuracy.