ISSN 0021-9665
10 papers in the library · 119 citations · publishing 1971-2025
Journal of Chromatographic Science January 1, 1973 John D. Wittwer, J. H. Kluckhohn 46 citations
A procedure to analyze d-lysergic acid diethylamide (LSD), the most widely abused hallucinogenic drug, incorporates a simple preliminary extraction step to isolate LSD from illicit preparations before analysis by high-speed liquid chromatography.
Journal of Chromatographic Science August 1, 1971 N. Narasimhachari, J. Spaide, Bh Heller 22 citations
N,N-dimethyltryptamines (DMT, 5-OMe-DMT, and bufotenin) were fully converted to trimethylsilyl derivatives with the TMS group attached to the indolic nitrogen. Gas-liquid chromatography data for these derivatives and mass spectrometry data from combined GC-MS analysis are reported. Secondary amines N-methyltryptamine and N-methylserotonin formed multiple derivatives, with the indolic NH reacting more readily than the secondary amino NH. Primary amines reacted with carbon disulfide to produce isothiocyanates that exhibit good gas chromatography properties and are well suited for GC-MS studies.
Journal of Chromatographic Science January 1, 1973 Ellen M. Faed, W. R. Mcleod 19 citations
A method for detecting lysergide (LSD-25) in urine is described, using solvent extraction and thin-layer chromatography. The procedure concentrates the drug from a 25 ml urine sample, hydrolyzes it to iso-lysergic acid, and then extracts and chromatographs the product. The limit of detection is approximately 0.5 micrograms per milliliter of urine. The test is intended for routine screening in clinical or forensic settings.
Journal of Chromatographic Science November 15, 2018 Norbert Rácz, Júlia Nagy, Wen Jiang et al. 12 citations
Three hydrophilic interaction liquid chromatography (HILIC) charge modulated amide columns—iHILIC®-Fusion, iHILIC®-Fusion(+), and iHILIC®-Fusion(P)—were compared for separating hallucinogenic alkaloids (psilocin, psilocybin, and baeocystin) from an extract of a truffle-like fungus. Three modeling methods predicted retention times in isocratic separation as a function of mobile phase composition, pH, and temperature. All columns separated the two hallucinogenic alkaloids from each other and from matrix components, with most compounds achieving satisfactory resolution. The quadratic modeling approach best predicted chromatograms under predefined conditions, the exponential model performed worst, and multivariate data analysis fell between the two.
Journal of Chromatographic Science May 1, 1974 Albert R. Sperling 10 citations
A method for identifying LSD combines thin-layer chromatography and gas chromatography. For gas chromatographic identification, the trimethylsilyl derivative of LSD is prepared to enable detection.
Journal of Chromatographic Science May 1, 2007 A. L. Thigpen, J. Deruiter, Charles R. Clark 9 citations
Six regioisomeric methylenedioxyphenethylamines, including three known drugs of abuse (MDEA, MDMMA, MBDB) and their 2,3-methylenedioxy counterparts, have nearly identical mass spectra and cannot be distinguished by electron impact mass spectrometry alone. Differentiation requires derivatization to pentafluoropropionylamides (PFPA) or heptafluorobutrylamides (HFBA). Underivatized amines are not resolved on non-polar stationary phases but are separated on a more polar trifluoropropylmethyl polysiloxane (Rtx-200) or a permethylated beta-cyclodextran (Rtx-bDEX) column. The PFPA and HFBA derivatives of the 2,3-methylenedioxyphenethylamines elute before the corresponding 3,4-methylenedioxyphenethylamine derivatives on the Rtx-200 column.
Journal of Chromatographic Science October 26, 2020 Júlia Nagy, Tibor Veress 1 citation
A mathematical model originally developed for supercritical fluid extraction of cannabinoids was tested for liquid extraction of other psychoactive compounds. The model successfully predicted extraction times and recoveries for quasi-counter current liquid-solid extractions of psilocin from hallucinogenic mushrooms, mescaline from cactus, harmine from a tropical liana, and salvinorin A from sage. Calculated component transport constants indicated extraction velocity: higher constants correspond to faster extraction. For mushrooms, pretreatment with liquid nitrogen markedly improved psilocin extractability.
Journal of Chromatographic Science November 15, 2025 Mahima Bansal, Estelle Miller, Rachael Sumner et al.
A new high-performance liquid chromatography method accurately measures LSD and separates it from its degradation product iso-LSD. Validated according to international guidelines, the method works even when LSD is exposed to stress conditions that cause breakdown. Applied to illicit microdosing samples from a New Zealand drug checking service, the analysis found a significant discrepancy between users' estimated doses and actual LSD levels. This highlights risks for people using non-pharmaceutical microdosing preparations and underscores the need for reliable quality control to ensure safety.
Journal of Chromatographic Science July 12, 2016 B. Duffau, Cristian Camargo, M. Kogan et al.
A new HPTLC method was developed and validated to identify and quantify the synthetic hallucinogen 25-C-NBOMe in seized blotters, with confirmation by GC–MS. Analysis of 15 real samples found 25-C-NBOMe in all cases, with dosages ranging from 701.0 to 1943.5 µg per blotter. The findings suggest that NBOMes are replacing LSD as the main hallucinogenic drug consumed in blotters in Chile, addressing a serious forensic, regulatory, and public health issue.
Journal of Chromatographic Science October 1, 2010 Tamer Awad, Tarek Belal, Hadir M Maher et al.
Three drugs of abuse—MDEA, MDMMA, and MBDB—and seven additional side chain regioisomers share the same molecular weight (207) and major mass spectral fragments (m/z 72 and 135/136), making them difficult to distinguish by routine mass spectrometry. The authors evaluated trifluoroacetyl, pentafluoropropionyl, and heptafluorobutryl derivatives of the primary and secondary amines using gas chromatography-mass spectrometry. These derivatives produced significantly individualized mass spectra with unique fragment ions that allowed specific side chain identification. Trifluoroacetyl and heptafluorobutryl derivatives provided the most specific ions for distinguishing the compounds, and all perfluoroacyl derivatives showed reasonable resolution on the polar Rtx-200 stationary phase.