An on-line immunoaffinity extraction-coupled column capillary liquid chromatography/tandem mass spectrometry (IAE/LC/LC/MS/MS) method is described. The system involves three columns, a 2.1-mm-i.d. protein G immunoaffinity column with noncovalently immobilized antibody specific to the analytes of interest, a packed capillary trapping column, and a packed capillary analytical column. With use of...
The in vitro metabolism of D-lysergic acid diethylamide (LSD) by human liver microsomes was investigated. Tandem mass spectrometric techniques, using precursor and neutral loss scans, were employed in the initial search for drug metabolites. The determination of LSD human liver in vitro metabolites was performed by high-performance liquid chromatography and capillary electrophoresis coupled...