Jack D. Henion

2 papers in the library · 183 citations · publishing 1996

Papers

On-Line Immunoaffinity Extraction-Coupled Column Capillary Liquid Chromatography/Tandem Mass Spectrometry: Trace Analysis of LSD Analogs and Metabolites in Human Urine

Analytical Chemistry January 1, 1996 Jianyi Cai, Jack D. Henion 112 citations

An on-line immunoaffinity extraction-coupled column capillary liquid chromatography/tandem mass spectrometry method was developed to detect LSD and its analogs and metabolites in human urine. The system uses three columns and allows direct analysis of diluted urine without pretreatment, eliminating sample handling steps. Concentrations as low as 2.5 parts per trillion of LSD and several analogs were detected in spiked human urine, which is 20-fold lower than the previous limit of detection using solid phase extraction and liquid chromatography/tandem mass spectrometry. The method was also applied to urine specimens from LSD users.

DOI: 10.1021/ac950763i Tandem mass spectrometry Detection limit Analyte Extraction chemistry +5

Elucidation of LSD In Vitro Metabolism by Liquid Chromatography and Capillary Electrophoresis Coupled with Tandem Mass Spectrometry*

Journal of Analytical Toxicology January 1, 1996 Jianyi Cai, Jack D. Henion 71 citations

The in vitro metabolism of LSD by human liver microsomes was investigated using tandem mass spectrometry, high-performance liquid chromatography, and capillary electrophoresis. Two new metabolites, lysergic acid ethylamide (LAE) and 2-oxo-LSD, were positively identified by comparison with reference standards. Other detected metabolites included mono- and trioxylated forms. The major metabolic route is deethylation. In human urine specimens from LSD users, iso-LSD was present at the highest concentration, followed by nor-LSD and isonor-LSD, with low levels of LAE and iso-LAE also found.

DOI: 10.1093/jat/20.1.27 Full text Lysergic acid diethylamide Chemistry Tandem mass spectrometry Urine +4