Development of a PCR Assay Targeting the iboH Gene to Assess the Distribution of Ibotenic Acid/Muscimol Biosynthesis Potential in the Genus Amanita
Journal of Fungi September 16, 2026 Zheng-Mi He, Jia-Xi Li
Ibotenic acid and muscimol, synthesized by the ibotenic acid biosynthetic gene cluster (ibo BGC) in Amanita species, are two of the primary neurotoxins responsible for mushroom poisoning incidents worldwide. However, the molecular detection methods for these toxins remain underdeveloped. In this study, we designed a pair of universal PCR primers targeting iboH, a hydroxylase-encoding gene...