Serotonin 2A Receptor (5-HT2AR) Activation by 25H-NBOMe Positional Isomers: In Vitro Functional Evaluation and Molecular Docking.
ACS Pharmacology & Translational Science February 25, 2021 E. Pottie, O. Kupriyanova, Asher L. Brandt et al.
Five positional isomers of the psychedelic compound 25H-NBOMe, each differing in the placement of two methoxy groups on the phenyl ring of the phenethylamine moiety, were tested in split-nanoluciferase assays to measure their ability to recruit cytosolic proteins to the serotonin 2A receptor. Molecular docking simulations estimated which receptor residues interact with each isomer's methoxy groups. This is the first comparative evaluation of how the positioning of methoxy groups in the phenethylamine moiety of NBOMes affects functional activity at the receptor.