Paper spray and direct mushroom spray mass spectrometry methods for the analysis of psycho-neurological disorder toxins
Wei Luo, Qiaolian Dai, Teris André van Beek, Han T. Zuilhof, Bo Chen, Zuohong Chen, Gert IJ. Salentijn
Food Chemistry July 2, 2025 DOI: 10.1016/j.foodchem.2025.145369 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Method development and validation study Peer reviewed |
|---|---|
| Population | Mushroom tissue and mushroom extract, including Amanita griseopantherina |
| Measures | AEPS-MS/MS, HPLC-MS/MS, solid-phase extraction coupled with PS-MS/MS |
| Citations | 3 |
| Key points | Direct paper-spray ionization identified muscarine, psilocin, and ibotenic acid from mushroom tissue within 2–3 minutes. The new AEPS-MS/MS method quantified ibotenic acid with a detection limit of 1.3 μg mL−1, accuracy of −14.0% to +5.1%, and precision of 10.8% to 18.0%, and its results in Amanita griseopantherina matched those from HPLC-MS/MS. |
Abstract
Fast screening approaches based on paper spray ionization were developed to identify psycho-neurological disorders mushrooms. Directly spraying from mushroom tissue already allowed direct identification of muscarine, psilocin, and ibotenic acid within 2–3 min. For quantitative analysis of the more challenging ibotenic acid, a new anion-exchange modified paper spray tandem mass spectrometry (AEPS-MS/MS) method was established to increase selectivity and sensitivity. The developed method was benchmarked against HPLC-MS/MS (with detection limits of 0.009 μg mL −1 ) and could reach detection limits of 1.3 μg mL −1 for ibotenic acid spiked in mushroom extract, with acceptable accuracy (−14.0 % to +5.1 %) and precision (10.8 % to 18.0 %). In addition, the developed method was compared with solid-phase extraction coupled with PS-MS/MS (with detection limits of 2.8 μg mL −1 ). Finally, the AEPS-MS/MS was applied for the quantitative analysis of ibotenic acid in Amanita griseopantherina , matching results from HPLC-MS/MS .
Citations in the library
Cites 2
- Determination of psilocybin and psilocin content in multiple Psilocybe cubensis mushroom strains using liquid chromatography - tandem mass spectrometry. Analytica Chimica Acta February 1, 2024
- Determination of ibotenic acid and muscimol in species of the genus Amanita section Amanita from China. Toxicon August 1, 2023