Activation of the cannabinoid receptor by delta 9-tetrahydrocannabinol reduces gamma-aminobutyric acid uptake in the globus pallidus.
Y P Maneuf, J E Nash, A R Crossman, J M Brotchie
European Journal of Pharmacology July 18, 1996 DOI: 10.1016/0014-2999(96)00326-3 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | In vitro study Peer reviewed |
|---|---|
| Population | Rat globus pallidus tissue slices |
| Interventions | delta 9-tetrahydrocannabinol SR 141716A |
| Measures | [3H]GABA uptake |
| Topics | Cannabis |
| Key points | Delta 9-THC reduced [3H]GABA uptake into rat globus pallidus slices in a concentration-dependent manner (51% decrease at 100 microM, IC50 = 18.95 microM). The cannabinoid receptor antagonist SR 141716A reversed this effect (IC50 = 11.9 microM) but had no effect on its own, which the authors interpret as evidence that cannabinoid receptor activation reduces GABA uptake in the globus pallidus. |
Abstract
The interaction between GABA (gamma-aminobutyric acid) and cannabinoids in the globus pallidus was investigated by evaluating the effects of delta 9-tetrahydrocannabinol on [3H]GABA uptake into slices of rat globus pallidus. delta 9-Tetrahydrocannabinol caused a concentration-dependent decrease in GABA uptake (51% decrease at 100 microM delta 9-tetrahydrocannabinol, IC50 = 18.95 microM). This effect was reversed in a concentration-dependent manner (IC50 = 11.9 microM) by the cannabinoid receptor antagonist SR 141716A (N-(piperidin-1-yl-)5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-me thyl-1 H-pyrazole-3-arboxiamidehydrochloride. SR 141716A alone did not affect GABA uptake. These results show that cannabinoid receptor activation reduces GABA uptake in the globus pallidus.
Comparable studies
Other preclinical and animal studies on cannabis, most cited first.
Citations in the library
Cited by 5
- Influence of tiagabine maintenance on cannabis effects and related behaviors in daily cannabis users. Experimental and Clinical Psychopharmacology June 2018
- Separate and combined effects of the GABA reuptake inhibitor tiagabine and Δ9-THC in humans discriminating Δ9-THC. Drug and Alcohol Dependence April 1, 2012
- Delta9-tetrahydrocannabinol is a full agonist at CB1 receptors on GABA neuron axon terminals in the hippocampus. Neuropharmacology 2010
- The effects of cannabinoids on the brain. Progress in Neurobiology July 1999
- The functional neuroanatomy of brain cannabinoid receptors. Neurobiology of Disease December 1998