3D‐based analyses of pharmacological modulation of zebrafish locomotion
Jonathan Cachat, Russell Reihl, Jessica Cosgrove, Siddharth Gaikwad, Jeremy Green, Peter C. Hart, Karoly Vollmer, Eli Utterback, Evan J. Kyzar, Kate Rhymes, Molly Hook, Katie Chang, Adam Stewart, Alan Newman, Alex Allain, Mimi Pham, Allan V. Kalueff
The FASEB Journal April 1, 2011 DOI: 10.1096/fasebj.25.1_supplement.1006.1 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Methods development study Peer reviewed |
|---|---|
| Population | Adult zebrafish |
| Interventions | Caffeine Morphine Ethanol Fluoxetine Nicotine Lysergic acid diethylamide 3 |
| Citations | 1 |
| Key points | The authors report that integrating manual observations, computerized event-based scoring, and automated video analysis into three-dimensional swim-path reconstruction improves characterization of complex anxiety-related behavior in adult zebrafish. They used caffeine and repeated morphine withdrawal as high-anxiety models and ethanol, morphine, fluoxetine, and nicotine as anxiolytic-like models, correlating multiple anxiety-like behaviors with manual observations, and applied the method to LSD and MDMA effects on novelty-evoked exploration. |
Abstract
Here, we integrated manual observations, computerized event‐based scoring and automated video analysis of zebrafish traces to generate three‐dimensional (3D) swim path reconstruction of general behavioral patterns, thereby improving the characterization and interpretation of complex, anxiety‐related adult zebrafish behavior. Drugs known to evoke a high‐anxiety state (caffeine, repeated morphine withdrawal) or an anxiolytic‐like effect (ethanol, morphine, fluoxetine, nicotine) wereused to detect multiple anxiety‐like behaviors and correlate them to those established manual observations. Additionally, we used this method to characterize the effects of lysergic acid diethylamide and 3,4‐methylenedioxymethamphetamine on zebrafish novelty‐evoked exploration. Our approach markedly enhances the power of zebrafish behavioral pharmacology, by providing innovative techniques for high‐throughput, multidimensional neurophenotyping of adult zebrafish behavior.