Cross-Reactivity of Amphetamine Analogues with Roche Abuscreen Radioimmunoassay Reagents
Journal of Analytical Toxicology 1990 DOI: 10.1093/jat/14.1.50 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Laboratory cross-reactivity study Peer reviewed |
|---|---|
| Population | Blank negative reference material spiked with amphetamine analogues |
| Measures | Abuscreen amphetamine radioimmunoassay |
| Citations | 22 |
| Key points | MDA was the only amphetamine analogue that showed cross-reactivity equal to or greater than amphetamine in the Abuscreen radioimmunoassay. None of the other tested analogues produced a positive result even at 100,000 ng/mL, though several showed depressed counts at various concentrations. |
Abstract
Cross-reactivity of amphetamine analogues with the Abuscreen amphetamine radioimmunoassay reagents was determined for both the standard and high specificity antibody systems. Compounds tested included 2-methoxyamphetamine, 4-hydroxymethamphetamine, 2,5-dimethoxyamphetamine (DMA), 4-bromo-2,5-dimethoxyamphetamine (DOB), 4-bromo-2,5-dimethoxy-beta-phenethylamine (BDMPEA), 3,4,5-trimethoxyamphetamine (TMA), 3,4-methylenedioxyamphetamine (MDA), N,N-dimethyl-3,4-methylenedioxyamphetamine and N-hydroxy-3,4-methylenedioxyamphetamine (N-OH MDA), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyethylamphetamine (MDEA), 2,5-dimethoxy-4-ethylamphetamine, 2,5-dimethoxy-4-methylamphetamine (DOM), and 3,4,5-trimethoxyphenethylamine (mescaline). Blank negative reference material was spiked with 1,000 to 100,000 ng/mL of the amphetamine analogue and used as sample in the assays. MDA was the only analogue that showed cross reactivity equal to or greater than that of amphetamine. None of the other analogue compounds demonstrated a positive result at even the highest concentration; however several showed depressed counts at various concentration levels.