Fate of mRNA following disaggregation of brain polysomes after administration of (+)-lysergic acid diethylamide in vivo
Bba Section Nucleic Acids and Protein Synthesis November 22, 1979 DOI: 10.1016/0005-2787(79)90092-3 (opens in new tab) via Elsevier
Summary
AI-generated from the abstractInjecting (+)-lysergic acid diethylamide into young rabbits caused a temporary breakdown of brain polysomes into monosomes, indicating inhibited protein synthesis. The messenger RNA (mRNA) remained intact and was not degraded or released; instead, it accumulated on monosomes. The mRNA coding for a brain-specific protein, S100, was preserved. The formation of a blocked monosome complex containing intact mRNA and ribosomal subunits but lacking nascent peptide chains suggests that (+)-lysergic acid diethylamide disrupts brain protein synthesis at a specific step during late initiation or early elongation.
Study at a glance
| Characteristics | Experimental study Peer reviewed |
|---|---|
| Population | Young rabbits |
| Intervention | (+)-lysergic acid diethylamide |
| Citations | 15 |
| Key finding | (+)-lysergic acid diethylamide inhibits brain protein synthesis by causing polysome disaggregation and accumulation of intact mRNA on blocked monosomes, likely at late initiation or early elongation. |
Abstract
Intravenous injection of (+)-lysergic acid diethylamide into young rabbits induced a transient brain-specific disaggregation of polysomes to monosomes. Investigation of the fate of mRNA revealed that brain poly(A+)mRNA was conserved. In particular, mRNA coding for brain-specific S100 protein was not degraded, nor was it released into free ribonucleoprotein particles. Following the (+)-lysergic acid diethylamide-induced disaggregation of polysomes, mRNA shifted from polysomes and accumulated on monosomes. Formation of a blocked monosome complex, which contained intact mRNA and 40-S plus 60-S ribosomal subunits but lacked nascent peptide chains, suggested that (+)-lysergic acid diethylamide inhibited brain protein synthesis at a specific stage of late initiation or early elongation.