Analysis of Serotonin in Caenorhabditis Elegans Subjected to Micro-Dosing with Psilocybin| Iris Publishers
Insights in Chemistry and Biochemistry October 15, 2025 DOI: 10.33552/icbc.2025.03.000562 (opens in new tab) via OpenAlex
Summary
AI-generated from the abstractA method was developed to measure serotonin in the roundworm Caenorhabditis elegans. The process involved extracting serotonin from basic solution into n-heptanol, back-extracting into acetic acid, then labeling with a fluorescent dye and cyanide. Capillary electrophoresis with laser-induced fluorescence detected serotonin at 1 μM. Worms were grown with or without psilocybin. Psilocybin reduced serotonin concentration from 0.9 to 0.5 mg/mg protein.
Study at a glance
| Characteristics | Experimental study Peer reviewed |
|---|---|
| Population | Caenorhabditis elegans |
| Intervention | psilocybin |
| Topics | Serotonin |
| Keywords | Caenorhabditis elegans Iris biosensor Capillary electrophoresis Fluorescence Molecular biology |
| Key finding | Psilocybin decreased serotonin concentration in C. elegans from 0.9 to 0.5 mg/mg protein. |
Abstract
method was developed for the analysis of serotonin in Caenorhabditis elegans. Samples were subjected to solvent-solvent extraction from basic conditions into n-heptanol. The serotonin was then back-extracted into acetic acid, the solution diluted into basic buffer, and labelled with 3-(2-furoyl) quinoline-2-carboxaldehyde in the presence of cyanide. The sample was analyzed by capillary electrophoresis utilizing post-column laser-induced fluorescence detection within a sheath flow cuvette. Under these conditions serotonin was detected at 1 M. C. elegans was cultured in the presence and absence of psilocybin. The presence of psilocybin was found to decrease the concentration of serotonin from 0.9 to 0.5 mg/mg protein.