A draft reference assembly of the Psilocybe cubensis genome
Kevin Mckernan, Liam T. Kane, Seth Crawford, Chen-Shan Chin, Aaron Trippe, Stephen Mclaughlin
F1000Research June 15, 2021 DOI: 10.12688/f1000research.51613.2 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Genome assembly Peer reviewed |
|---|---|
| Population | Psilocybe cubensis mushroom |
| Keywords | Contig Genome Computational biology Genetics Alkaloids |
| Citations | 10 |
| Key points | The genome of Psilocybe cubensis was assembled into 32 contigs, with the psilocybin synthesis cluster located on a single 3.2 megabase contig. |
Abstract
We describe the use of high-fidelity single molecule sequencing to assemble the genome of the psychoactive Psilocybe cubensis mushroom. The genome is 46.6Mb, 46% GC, and in 32 contigs with an N50 of 3.3Mb. The BUSCO completeness scores are 97.6% with 1.2% duplicates. The Psilocybin synthesis cluster exists in a single 3.2Mb contig. The dataset is available from NCBI BioProject with accessions PRJNA687911 and PRJNA700437.
Citations in the library
Cited by 8
- A journey with psychedelic mushrooms: From historical relevance to biology, cultivation, medicinal uses, biotechnology, and beyond Biotechnology Advances August 31, 2023
- Phylogenomics of the psychoactive mushroom genus Psilocybe and evolution of the psilocybin biosynthetic gene cluster Proceedings of the National Academy of Sciences January 9, 2024
- Genome sequencing progenies of magic mushrooms (Psilocybe subaeruginosa) identifies tetrapolar mating and gene duplications in the psilocybin pathway Fungal Genetics and Biology December 29, 2022
- Exploring Psilocybe cubensis Strains: Cultivation Techniques, Psychoactive Compounds, Genetics and Research Gaps Journal of Fungi January 28, 2025
- Exploring Psilocybe spp. mycelium and fruiting body chemistry for potential therapeutic compounds Frontiers in Fungal Biology November 29, 2023
and 3 more in the library
Cites 11
- Potential Therapeutic Effects of Psilocybin. Neurotherapeutics July 1, 2017
- Enzymatic Synthesis of Psilocybin Angewandte Chemie International Edition August 1, 2017
- Horizontal gene cluster transfer increased hallucinogenic mushroom diversity Evolution Letters February 27, 2018
- Simultaneous Production of Psilocybin and a Cocktail of β‐Carboline Monoamine Oxidase Inhibitors in “Magic” Mushrooms Chemistry - A European Journal November 14, 2019
- Production Options for Psilocybin: Making of the Magic Chemistry - A European Journal July 16, 2018
and 6 more in the library