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Methods in molecular biology (Clifton, N.J.)

ISSN 1940-6029

4 papers in the library · 1 citation · publishing 2018-2025

Papers

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Unveiling Ketamine's Influence on Astrocytic Kir4.1 Channels Through Multimodal Analysis: Confocal Microscopy, Immunocytochemistry, Fluorescence Analysis, and Electrophysiology.

Methods in molecular biology (Clifton, N.J.) 2025 Samo Pirnat, Katja Fink, Matjaž Stenovec et al.

Understanding the elusive mechanisms responsible for the therapeutic efficacy of ketamine in major depressive disorder (MDD) is crucial. Astrocytes play a vital role in regulating extracellular potassium concentration ([K+]o), which is essential for maintaining proper neuronal excitability and overall brain function. Dysregulation of [K+]o can lead to significant changes in neuronal activity,...

Quantification of Phencyclidine (PCP) in Urine, Serum, or Plasma by Gas Chromatography-Mass Spectrometry (GC-MS).

Methods in molecular biology (Clifton, N.J.) 2024 David Scott, C Clinton Frazee, Uttam Garg

Phencyclidine (PCP), a dissociative anesthetic, is a commonly abused recreational drug. In the 1950s, initially tested as an intravenous anesthetic, PCP was discontinued for clinical use due to its severe adverse effects. Since then, it has gained popularity as a recreational drug due to its ability to induce hallucinations and alter perception. PCP can be detected in urine, serum, or plasma by...

Evaluation of Sensorimotor Gating Deficits in Mice Through Prepulse Inhibition (PPI) of the Startle Response.

Methods in molecular biology (Clifton, N.J.) 2023 Paula Unzueta-Larrinaga, Leyre Urigüen 1 citation

Prepulse inhibition of the startle response enables measuring animal behavior and helps us understand core aspects of neuropsychiatric diseases. Prepulse inhibition is considered a translational indicator of sensorimotor gating deficits present in schizophrenia patients and is crucial in the characterization of animal models of schizophrenia-like behaviors. Hallucinogenic drugs acting through...

Hyperlocomotion Test for Assessing Behavioral Disorders.

Methods in molecular biology (Clifton, N.J.) 2018 Dan Ma, Paul C Guest

Under- or overfeeding during pregnancy can lead to behavioral deficits in the offspring in later life. Here, we present a protocol for setting up and carrying out the hyperlocomotion test for assessing behavioral symptoms such as psychosis or mania. As an example, we use the acute rat phencyclidine-injection model which exhibits hyperlocomotion and stereotypic behaviors, resembling the positive...