A method for the determination of hordenine, gramine, 5-methoxy-N,N-dimethyltryptamine and N-methyltryptamine in reed canarygrass is described. The material is chopped into pieces, 0.5–1 cm, and extracted overnight in 20% ethanol and 1% acetic acid. The extract is purified by solid phase extraction using a strong cation exchange column. Interferences are removed by selective elution with water...