A Method for the Determination of Alkaloids in Reed Canarygrass (Phalaris arundinaceaeL.) using HPLC and Solid Phase Extraction
Gunnar Kalén, Robert Samuelsson, Eva Törmä
Acta Agriculturae Scandinavica Section B - Soil & Plant Science December 1, 1992 DOI: 10.1080/09064719209410216 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Method development study Peer reviewed |
|---|---|
| Population | Reed canarygrass, including the variety Palaton |
| Citations | 3 |
| Key points | The described extraction and HPLC method recovered 74 to 85% of hordenine, gramine, 5-methoxy-N,N-dimethyltryptamine and N-methyltryptamine from reed canarygrass, and alkaloid concentrations measured in the variety Palaton agreed with earlier reports. |
Abstract
A method for the determination of hordenine, gramine, 5-methoxy-N,N-dimethyltryptamine and N-methyltryptamine in reed canarygrass is described. The material is chopped into pieces, 0.5–1 cm, and extracted overnight in 20% ethanol and 1% acetic acid. The extract is purified by solid phase extraction using a strong cation exchange column. Interferences are removed by selective elution with water and methanol. The alkaloids are eluted with 1 M potassium acetate in methanol and the analysis is carried out by HPLC and UV detection. The column used was Kromasil C18 and the eluent comprised 30% methanol, 1 mM N,N-dimethyloctylamine, 10 mM sodium heptansulfonic acid and 25 mM citrate buffer, pH 3.1. The influence of eluent composition, together with extraction and purification parameters, was investigated. The alkaloids recovered by the extraction and determination methods ranged from 74 to 85% and the alkaloid concentration determined in the variety Palaton was in good agreement with earlier reports.