Sensitive determination of MDMA and its metabolite MDA in rat blood and brain microdialysates by HPLC with fluorescence detection
Biomedical Chromatography May 2, 2007 Mamoru Tomita, Mihoko N. Nakashima, Mitsuhiro Wada et al. 22 citations
A method using high-performance liquid chromatography with fluorescence detection was developed to simultaneously measure MDMA and its metabolite MDA in rat blood and brain microdialysates. The technique, which involves derivatization with DIB-Cl, achieved linear calibration curves for MDA and MDMA over ranges of 2.5–500 and 5.0–1000 ng/mL, respectively. Detection limits were 1.2 ng/mL for MDA and 4.2 ng/mL for MDMA in blood, and 1.3 and 4.8 ng/mL in brain. Precision was under 5.6%. The method was used to monitor MDMA and MDA in rats and to evaluate pharmacokinetic parameters after MDMA administration with or without caffeine.