A rapid method using direct analysis in real time-high-resolution mass spectrometry (DART-HRMS) quantifies N,N-dimethyltryptamine (DMT) in ayahuasca brews without lengthy sample preparation. The technique also identifies the beverage by detecting secondary metabolites from its plant ingredients. Analysis of six ayahuasca brews made with different combinations of DMT- and harmala alkaloid-containing plants found DMT levels ranging from 45.7 to 230.5 mg/L, consistent with values from conventional methods.
A direct analysis in real time-high-resolution mass spectrometry (DART-HRMS) method rapidly screens and quantifies salvinorin A in Salvia divinorum plant material without lengthy sample pretreatment. Raw Salvia leaves contained an average of 1.54 mg/g of salvinorin A, while enhanced leaf extracts had between 13.0 and 53.2 mg/g. Cholesterol served as a suitable internal standard for quantification. The approach bypasses traditional chromatographic sample preparation, enabling faster assessment of psychoactive plant materials.