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Gunnar Kalén

1 paper in the library · 3 citations · publishing 1992

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A Method for the Determination of Alkaloids in Reed Canarygrass (Phalaris arundinaceaeL.) using HPLC and Solid Phase Extraction

Acta Agriculturae Scandinavica Section B - Soil & Plant Science December 1, 1992 Gunnar Kalén, Robert Samuelsson, Eva Törmä 3 citations

A method for the determination of hordenine, gramine, 5-methoxy-N,N-dimethyltryptamine and N-methyltryptamine in reed canarygrass is described. The material is chopped into pieces, 0.5–1 cm, and extracted overnight in 20% ethanol and 1% acetic acid. The extract is purified by solid phase extraction using a strong cation exchange column. Interferences are removed by selective elution with water...