Identification of Metabolite Biomarkers of the Designer Hallucinogen 25I-NBOMe in Mouse Hepatic Microsomal Preparations and Human Urine Samples Associated with Clinical Intoxication.
Journal of Analytical Toxicology October 1, 2015 Justin L. Poklis, Sara K Dempsey, Kai Liu et al.
Fifteen metabolites of the designer hallucinogen 25I-NBOMe were identified in mouse liver microsomal preparations and in urine from two intoxicated patients. One patient's urine contained the parent drug and all fifteen metabolites; the other contained only three O-desmethyl metabolites. Two major urinary metabolites were synthesized. The authors recommend using β-glucuronidase hydrolysis before screening and using the metabolite M5 as the primary biomarker for detecting 25I-NBOMe use.