25C-NBOMe and 25I-NBOMe metabolite studies in human hepatocytes, in vivo mouse and human urine with high-resolution mass spectrometry.
Drug Testing and Analysis May 1, 2017 Ariane Wohlfarth, Markus Roman, Mikael Andersson et al.
The hallucinogenic drugs 25C-NBOMe and 25I-NBOMe are primarily broken down in the body by O-demethylation, followed by O-di-demethylation and hydroxylation. All methoxy groups could be demethylated; hydroxylation occurred mainly on the NBOMe ring. Phase I metabolites were extensively conjugated with glucuronic acid and sulfate in human urine. Specific and abundant urine targets for detecting 25C-NBOMe are 5'-desmethyl 25C-NBOMe, 25C-NBOMe, and 5-hydroxy 25C-NBOMe; for 25I-NBOMe, they are 2' and 5'-desmethyl 25I-NBOMe and hydroxy 25I-NBOMe. These findings can aid clinical and forensic laboratories in developing analytical methods and interpreting results.