Donald. E. Brooks

2 papers in the library · 9 citations · publishing 1998

Papers

Indirect enzyme-linked immunosorbent assay for the quantitative estimation of lysergic acid diethylamide in urine

Clinical Chemistry May 1, 1998 Sarah Kerrigan, Donald. E. Brooks 6 citations

A new antibody targeting LSD enabled the development of an indirect ELISA that measures LSD in urine with better performance than a commercial assay. The test uses 50 μL of urine and detects concentrations from ng/L to μg/L. The limit of detection is 8 ng/L, compared to 85 ng/L for the commercial assay, and analytical recoveries range from 98–106%. At 0.1 μg/L, the intraassay coefficient of variation (CV) was 2.4% (n=8), versus 6.0% at 0.5 μg/L for the commercial assay (n=20). The upper and lower quantification limits are 7 μg/L and 50 ng/L. Cross-reactivity with 24 related substances was evaluated. The new assay offers improved sensitivity and precision, enabling more certain quantitative estimation of LSD in urine at lower concentrations.

DOI: 10.1093/clinchem/44.5.985

Optimization and immunological characterization of a photochemically coupled lysergic acid diethylamide (LSD) immunogen

Bioconjugate Chemistry January 1, 1998 Sarah Kerrigan, Donald. E. Brooks 3 citations

A photoreactive linker was used to attach lysergic acid diethylamide (LSD) to a carrier protein, keyhole limpet hemocyanin, at multiple sites on the drug. About 35 drug molecules were attached per protein when equal amounts of both were used. Reaction efficiency was severely reduced by buffer components or water. Excess linker, pH, irradiation of the dry matrix without buffer, and the drug-to-protein ratio during photolysis strongly affected efficiency. Antibody specificity data suggested that the drug attached to the protein at positions N1 and N6 of LSD, consistent with the reactivity of the photogenerated aryl nitrene.

DOI: 10.1021/bc9800320 Full text