Phencyclidine-induced expression of c-Fos-like immunoreactivity in mouse brain regions.
S Sugita, M Namima, Toshitaka Nabeshima, K Okamoto, H Furukawa, Y Watanabe
Neurochemistry International 1996 DOI: 10.1016/0197-0186(95)00127-1 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Animal experimental study Peer reviewed |
|---|---|
| Population | Mice |
| Intervention | Phencyclidine |
| Dose | 5.0 mg/kg, i.p. |
| Duration | Up to 1 week after injection |
| Measures | c-Fos-like immunoreactivity, c-Jun-like immunoreactivity, locomotor activity |
| Key findings | A single 5.0 mg/kg intraperitoneal phencyclidine injection increased locomotor activity and c-Fos-like immunoreactivity in the parietal cortex, hippocampal dentate gyrus, piriform cortex, and hypothalamus. c-Fos-like immunoreactivity in the parietal cortex continued to increase for one week after injection. The authors suggest persistent c-Fos expression may underlie phencyclidine's behavioral or psychotomimetic effects. |
Abstract
For the purpose of studying a role of immediate early genes in psychotomimetic-induced behavioral excitation, we experimentally enhanced the locomotor activity of mice by acute administration of phencyclidine and examined the expression and localization of the c-Fos-like and c-Jun-like immunoreactivities in brain regions. A single injection of phencyclidine (5.0 mg/kg, i.p.) significantly increased not only the locomotor activity but also the expression of c-Fos-like immunoreactivity in several brain regions, particularly in the parietal cortex, hippocampal dentate gyrus, piriform cortex and hypothalamus. Interestingly, the c-Fos-like immunoreactivity in the parietal cortex continued to increase for 1 week after the phencyclidine injection. These results indicate that phencyclidine, even injected only once, can induce the persistent expression of c-Fos or c-Fos-related protein(s) in the mouse brain, and also suggest the possibility that such a c-Fos expression may underlie the behavioral and/or psychotomimetic effects of phencyclidine.