Phylogenetic relationship of psychoactive fungi based on the rRNA gene for a large subunit and their identification using the TaqMan assay.
Takuro Maruyama, Kazumasa Yokoyama, Yukiko Makino, Yukihiro Goda
Chemical & pharmaceutical bulletin June 1, 2003 DOI: 10.1248/cpb.51.710 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | Peer reviewed |
|---|---|
| Population | Several kinds of psychoactive "magic mushrooms" (MMs) |
| Keywords | Agaricales Rna, fungal Hallucinogens Phylogeny Species specificity Base sequence Genes, fungal Genes, rrna Molecular sequence data |
| Key points | The authors describe a phylogenetic analysis of psychoactive "magic mushrooms" based on a partial sequence (about 970 bp) of the rRNA large subunit gene, and report a TaqMan PCR assay for their rapid identification, an approach they propose because morphological identification is difficult when the fungi are sold as dried powders. |
Abstract
"Magic mushrooms" (MMs) are psychoactive fungi containing the and Psychotropics Control Law in Japan. Because there are many kinds of MMs and they are often sold even as dry powders in local markets, it is very difficult to identify the original species of the MMs by morphological observation. Therefore, we investigated the rRNA gene for a large subunit (LSU) of several MMs to classify them by a genetic approach. In this paper, we described the phylogeny of species of MMs based on the partial sequence (about 970 bp) of the LSU and the rapid identification of MMs using the TaqMan PCR assay.
Citations in the library
Cited by 5
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- [Chemical and DNA analyses for the products of a psychoactive plant, Voacanga africana]. Yakugaku Zasshi August 1, 2009
- Molecular and morphological studies reveal a new species of Panaeolus (Agaricales, Basidiomycota) from Punjab, Pakistan European Journal of Taxonomy August 10, 2023
- Phylogenetic relationship of psychoactive fungi based on rRNA gene for a large subunit and their identification using the TaqMan assay (II). Forensic Science International November 10, 2006