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Phylogenetic relationship of psychoactive fungi based on rRNA gene for a large subunit and their identification using the TaqMan assay (II).

Takuro Maruyama, Nobuo Kawahara, Kazumasa Yokoyama, Yukiko Makino, Toshimitsu Fukiharu, Yukihiro Goda

Forensic Science International November 10, 2006 DOI: 10.1016/j.forsciint.2004.10.028 (opens in new tab)

Study at a glance

AI-extracted from the abstract
Characteristics Theoretical or philosophical paper Peer reviewed
Population Two Panaeolus species and four Psilocybe species of psychoactive fungi, including two non-psilocybin species
Key points The authors report that they improved their previous identification system using ITS and LSU rRNA gene sequences, producing a new system capable of distinguishing magic mushrooms from non-psilocybin Psilocybe species. They also present application data and the phylogeny of magic mushroom species.

Abstract

"Magic mushroom (MM)" is the name most commonly given to psychoactive fungi containing the hallucinogenic components: psilocin (1) and psilocybin (2). We investigated the rRNA gene (internal transcribed spacer (ITS) and large subunit (LSU)) of two Panaeolus species and four Psilocybe species fungi (of these, two are non-psilocybin species). On the basis of sequence alignment, we improved the identification system developed in our previous study. In this paper, we describe the new system capable of distinguishing MMs from non-psilocybin Psilocybe species, its application data and the phylogeny of MM species.

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