ATG5 expression induced by MDMA (ecstasy), interferes with neuronal differentiation of neuroblastoma cells.
Myounghee Chae, Gyu-Seek Rhee, Ik-Soon Jang, Kwangsoo Kim, Ji-Hae Lee, Seung-Yeul Lee, Minjung Kim, Junyoung Yang, Junsoo Park, Seung-Hoon Lee
Molecules and Cells May 31, 2009 DOI: 10.1007/s10059-009-0075-2 (opens in new tab)
Study at a glance
AI-extracted from the abstract| Characteristics | In vitro experimental study Peer reviewed |
|---|---|
| Population | Whole mouse embryos and mouse/human neuroblastoma cell lines (SH-SY5Y and PC12 cells) |
| Intervention | MDMA |
| Measures | quantitative real-time PCR |
| Topics | MDMA |
| Key findings | MDMA treatment elevated Atg5 expression in mouse embryos and neuroblastoma cells, and this elevated Atg5 interfered with neuronal differentiation of SH-SY5Y and PC12 cells. The authors suggest that MDMA use during pregnancy may impair neuronal development via induction of Atg5 expression. |
Abstract
The amphetamine derivative 3, 4-methylenedioxymethamphetamine (MDMA) has become a popular recreational drug, and has also been shown to cause serotonergic neurotoxicity. This report shows that MDMA impairs brain development in a whole mouse embryo culture. The results of quantitative real-time PCR analysis showed that autophagy-related protein 5 (Atg5) expression is elevated in mouse embryo and neuroblastoma cells after MDMA treatment. This elevated Atg5 expression interferes with the neuronal differentiation of neuroblastoma cells such as SH-SY5Y and PC12 cells. Thus, our results suggest that the use of MDMA during pregnancy may impair neuronal development via an induction of Atg5 expression.
Comparable studies
Other preclinical and animal studies on MDMA, most cited first.
Citations in the library
Cited by 5
- Autophagy activation is involved in 3,4-methylenedioxymethamphetamine ('ecstasy')--induced neurotoxicity in cultured cortical neurons. PLoS One December 31, 2014
- Of mice and men on MDMA: a translational comparison of the neuropsychobiological effects of 3,4-methylenedioxymethamphetamine ('Ecstasy'). Brain Research November 14, 2019
- Autophagy inhibition plays a protective role against 3, 4‐methylenedioxymethamphetamine (MDMA)‐induced loss of serotonin transporters and depressive‐like behaviors in rats Pharmacological Research February 28, 2019
- MDMA-induced neurotoxicity of serotonin neurons involves autophagy and rilmenidine is protective against its pathobiology. Neurochemistry International May 2017
- Neurotoxicity of "ecstasy" and its metabolites in human dopaminergic differentiated SH-SY5Y cells. Toxicology Letters February 4, 2013
Cites 3
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- Prenatal 3,4-methylenedioxymethamphetamine (ecstasy) exposure induces long-term alterations in the dopaminergic and serotonergic functions in the rat. Brain research. Developmental brain research February 8, 2005
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